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Goat anti-NEUROD2 Antibody

EB07090
Everest Biotech
ApplicationsELISA
Product group Antibodies
ReactivityBovine, Canine, Human, Mouse, Rat
TargetNEUROD2
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-NEUROD2 Antibody
  • Delivery Days Customer
    5
  • Applications
    ELISA
  • Applications Supplier
    Pep-ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID4761
  • Target name
    NEUROD2
  • Target description
    neuronal differentiation 2
  • Target synonyms
    DEE72, EIEE72, NDRF, bHLHa1, neurogenic differentiation factor 2, class A basic helix-loop-helix protein 1, neuroD-related factor, neurogenic basic-helix-loop-helix protein, neurogenic differentiation 2
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_006151.3. Purification: Antigen affinity purified. Names and symbols: NEUROD2; neurogenic differentiation 2; HGNC:7763; NDRF; MGC26304; neuroD-related factor; neurogenic basic-helix-loop-helix protein; neurogenic differentiation factor 2; bHLHa1
  • Reactivity
    Bovine, Canine, Human, Mouse, Rat
  • Reactivity Supplier
    Human, Mouse, Rat, Dog, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NEUROD2 using anti-NEUROD2 antibody (A07904-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: rat C6 whole cell lysates, Lane 3: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NEUROD2 antigen affinity purified polyclonal antibody (Catalog # A07904-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NEUROD2 at approximately 50 kDa. The expected band size for NEUROD2 is at 41 kDa.
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