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NeuroD2 Antibody (YA2369, Rabbit)

HY-P82624
MedChem Express
TargetNEUROD2
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    NeuroD2 Antibody (YA2369, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID4761
  • Target name
    NEUROD2
  • Target description
    neuronal differentiation 2
  • Target synonyms
    DEE72, EIEE72, NDRF, bHLHa1, neurogenic differentiation factor 2, class A basic helix-loop-helix protein 1, neuroD-related factor, neurogenic basic-helix-loop-helix protein, neurogenic differentiation 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    NeuroD2 Antibody (YA2369) is a non-conjugated IgG antibody, targeting NeuroD2, with a predicted molecular weight of 41 kDa (observed band size: 48 kDa). NeuroD2 Antibody (YA2369) can be used for WB experiment in human, mouse, rat background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NEUROD2 using anti-NEUROD2 antibody (A07904-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: rat C6 whole cell lysates, Lane 3: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NEUROD2 antigen affinity purified polyclonal antibody (Catalog # A07904-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NEUROD2 at approximately 50 kDa. The expected band size for NEUROD2 is at 41 kDa.
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