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Neuro D2 Recombinant Antibody

BSM-62086R
Bioss
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetNEUROD2
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Overview

  • Supplier
    Bioss
  • Product Name
    Neuro D2 Recombinant Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Applications Supplier
    WB(1:300-5000)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID4761
  • Target name
    NEUROD2
  • Target description
    neuronal differentiation 2
  • Target synonyms
    DEE72, EIEE72, NDRF, bHLHa1, neurogenic differentiation factor 2, class A basic helix-loop-helix protein 1, neuroD-related factor, neurogenic basic-helix-loop-helix protein, neurogenic differentiation 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15784
  • Protein Name
    Neurogenic differentiation factor 2
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NEUROD2 using anti-NEUROD2 antibody (A07904-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: rat C6 whole cell lysates, Lane 3: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NEUROD2 antigen affinity purified polyclonal antibody (Catalog # A07904-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NEUROD2 at approximately 50 kDa. The expected band size for NEUROD2 is at 41 kDa.
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