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Western Blot analysis of Jurkat cells using Neuro D2 Polyclonal Antibody
Western Blot analysis of Jurkat cells using Neuro D2 Polyclonal Antibody
Western Blot analysis of Jurkat cells using Neuro D2 Polyclonal Antibody

NEUROD2 Antibody

CSB-PA003412
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetNEUROD2
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Overview

  • Supplier
    Cusabio
  • Product Name
    NEUROD2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4761
  • Target name
    NEUROD2
  • Target description
    neuronal differentiation 2
  • Target synonyms
    DEE72, EIEE72, NDRF, bHLHa1, neurogenic differentiation factor 2, class A basic helix-loop-helix protein 1, neuroD-related factor, neurogenic basic-helix-loop-helix protein, neurogenic differentiation 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15784
  • Protein Name
    Neurogenic differentiation factor 2
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NEUROD2 using anti-NEUROD2 antibody (A07904-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: rat C6 whole cell lysates, Lane 3: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NEUROD2 antigen affinity purified polyclonal antibody (Catalog # A07904-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NEUROD2 at approximately 50 kDa. The expected band size for NEUROD2 is at 41 kDa.
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