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ProductsBack/NFE2L2 / NRF2 Antibody

NFE2L2 / NRF2 Antibody

LS-C746748
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
TargetNFE2L2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    NFE2L2 / NRF2 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 65kDa/66kDa/67kDa, while the observed MW by Western blot was 110kDa.
  • Applications
    ImmunoFluorescence, Western Blot
  • Applications Supplier
    IF (1:50 - 1:200), WB (1:500 - 1:2000) The predicted MW is 65kDa/66kDa/67kDa, while the observed MW by Western blot was 110kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID4780
  • Target name
    NFE2L2
  • Target description
    nuclear factor, erythroid 2 like 2
  • Target synonyms
    HEBP1; IMDDHH; NRF2; Nrf-2; nuclear factor erythroid 2-related factor 2; nuclear factor erythroid-derived 2-like 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NFE2L2 using anti-NFE2L2 antibody (A00078-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human SW620 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFE2L2 antigen affinity purified polyclonal antibody (Catalog # A00078-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NFE2L2 at approximately 100KD. The expected band size for NFE2L2 is at 100KD.
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