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Goat anti-PTEN

EB06544
Everest Biotech
ApplicationsImmunoPrecipitation, Western Blot, ELISA
Product group Antibodies
TargetPTEN
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-PTEN Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Immunoprecipitation:This antibody was deemed fit for IP under native conditions (observations from a customer). Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
  • Applications
    ImmunoPrecipitation, Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB, IP
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID5728
  • Target name
    PTEN
  • Target description
    phosphatase and tensin homolog
  • Target synonyms
    10q23del; BZS; CWS1; DEC; GLM2; MHAM; mitochondrial phosphatase and tensin protein alpha; mitochondrial PTENalpha; MMAC1; MMAC1 phosphatase and tensin homolog deleted on chromosome 10; mutated in multiple advanced cancers 1; phosphatase and tensin-like protein; phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN; phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN; protein tyrosine phosphatase; PTEN variant PTEN-K267Rfs*9; PTEN variant PTEN-L247*; PTEN variant PTEN-R234Afs*11; PTEN1; PTENbeta; PTENepsilon; TEP1
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_000305.3. Purification: Antigen affinity purified. Names and symbols: PTEN; BZS; MHAM; TEP1; MMAC1; PTEN1; MGC11227; phosphatase and tensin homolog (mutated in multiple advanced cancers 1); tensin homolog; mutated in multiple advanced cancers 1; MMAC1 phosphatase and tension homolog deleted on chromosome 10; phosphatase and
  • Reactivity Supplier
    Human, Mouse, Rat, Dog, Pig, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • A high-throughput pipeline for validation of antibodies. Sikorski K et al., 2018 Nov, Nat Methods
    Read more

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Figure 1. Western blot analysis of PTEN using anti-PTEN antibody (A00006-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysate,Lane 2: mouse liver tissue lysate,Lane 3: human A549 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTEN antigen affinity purified polyclonal antibody (Catalog # A00006-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTEN at approximately 54KD. The expected band size for PTEN is at 47KD.
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