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Paraformaldehyde-fixed, paraffin embedded Rat lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PTEN Polyclonal Antibody, Unconjugated (bs-0686R) at 1:500 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PTEN Polyclonal Antibody, Unconjugated (bs-0686R) at 1:500 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PTEN Polyclonal Antibody, Unconjugated (bs-0686R) at 1:500 overnight at 4°C, DAB staining.

PTEN Polyclonal Antibody

BS-0686R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetPTEN
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Overview

  • Supplier
    Bioss
  • Product Name
    PTEN Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID5728
  • Target name
    PTEN
  • Target description
    phosphatase and tensin homolog
  • Target synonyms
    10q23del, BZS, CWS1, DEC, GLM2, MHAM, MMAC1, PTEN1, PTENbeta, PTENgama, TEP1, phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN, MMAC1 phosphatase and tensin homolog deleted on chromosome 10, PTEN variant PTEN-K267Rfs*9, PTEN variant PTEN-L247*, PTEN variant PTEN-R234Afs*11, PTENepsilon, inositol polyphosphate 3-phosphatase, mitochondrial PTENalpha, mitochondrial phosphatase and tensin protein alpha, mutated in multiple advanced cancers 1, phosphatase and tensin-like protein, phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN, protein tyrosine phosphatase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP60484
  • Protein Name
    Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • He Z, Elbaz A, Gao B, et al. Disposable Morpho menelaus Based Flexible Microfluidic and Electronic Sensor for the Diagnosis of Neurodegenerative Disease. Adv Healthc Mater. 2018,7(5). doi: 10.1002/adhm.201701306
    Read this paper
  • Li L, Cui J, Liu Z, et al. Silver nanoparticles induce SH-SY5Y cell apoptosis via endoplasmic reticulum- and mitochondrial pathways that lengthen endoplasmic reticulum-mitochondria contact sites and alter inositol-3-phosphate receptor function. Toxicol Lett. 2018,285:156-167. doi: 10.1016/j.toxlet.2018.01.004
    Read this paper

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Figure 1. Western blot analysis of PTEN using anti-PTEN antibody (A00006-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysate,Lane 2: mouse liver tissue lysate,Lane 3: human A549 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTEN antigen affinity purified polyclonal antibody (Catalog # A00006-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTEN at approximately 54KD. The expected band size for PTEN is at 47KD.
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