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PTEN Antibody (YA119, Rabbit)

HY-P80286
MedChem Express
TargetPTEN
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    PTEN Antibody (YA119, Rabbit)
  • Delivery Days Customer
    10
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID5728
  • Target name
    PTEN
  • Target description
    phosphatase and tensin homolog
  • Target synonyms
    10q23del; BZS; CWS1; DEC; GLM2; MHAM; mitochondrial phosphatase and tensin protein alpha; mitochondrial PTENalpha; MMAC1; MMAC1 phosphatase and tensin homolog deleted on chromosome 10; mutated in multiple advanced cancers 1; phosphatase and tensin-like protein; phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN; phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN; protein tyrosine phosphatase; PTEN variant PTEN-K267Rfs*9; PTEN variant PTEN-L247*; PTEN variant PTEN-R234Afs*11; PTEN1; PTENbeta; PTENepsilon; TEP1
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    PTEN Antibody (YA119) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PTEN.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PTEN using anti-PTEN antibody (A00006-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysate,Lane 2: mouse liver tissue lysate,Lane 3: human A549 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTEN antigen affinity purified polyclonal antibody (Catalog # A00006-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTEN at approximately 54KD. The expected band size for PTEN is at 47KD.
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