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ProductsBack/Goat anti-RAD51D / RAD51L3

Goat anti-RAD51D / RAD51L3

EB05692
Everest Biotech
ApplicationsELISA
Product group Antibodies
TargetRAD51D
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-RAD51D / RAD51L3 Antibody
  • Delivery Days Customer
    5
  • Applications
    ELISA
  • Applications Supplier
    Pep-ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID5892
  • Target name
    RAD51D
  • Target description
    RAD51 paralog D
  • Target synonyms
    BROVCA4; DNA repair protein RAD51 homolog 4; R51H3; RAD51 homolog D; RAD51L3; RAD51-like protein 3; recombination repair protein; TRAD
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_002869.3; NP_598332.1; NP_001136043.1. Purification: Antigen affinity purified. Names and symbols: RAD51L3; RAD51-like 3 (S. cerevisiae); TRAD; R51H3; HsTRAD; RAD51D; recombination repair protein; DNA repair protein RAD51 homolog 4; Trad; RAD51-like 3
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RAD51D using anti-RAD51D antibody (A02893-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human A2780 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAD51D antigen affinity purified polyclonal antibody (Catalog # A02893-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAD51D at approximately 30-40 kDa. The expected band size for RAD51D is at 35 kDa.
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