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ProductsBack/Goat anti-SM22 Alpha / Transgelin

Goat anti-SM22 Alpha / Transgelin

EB06281
Everest Biotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetTAGLN
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-SM22 Alpha / Transgelin Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID6876
  • Target name
    TAGLN
  • Target description
    transgelin
  • Target synonyms
    SM22, SM22-alpha, SMCC, TAGLN1, TGLN, WS3-10, transgelin, 22 kDa actin-binding protein, epididymis secretory sperm binding protein, smooth muscle protein 22-alpha
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_003177.2. GeneIDs all Nonhuman: 21345 (mouse); 25123 (rat);. Purification: Antigen affinity purified. Names and symbols: TAGLN; SM22; SMCC; WS3-10; transgelin; SM22-alpha; DKFZp686P11128; TAGLN1; DKFZp686B01212; transgelin variant 2
  • Reactivity Supplier
    Human, Mouse, Rat, Dog, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Bruno G, Cencetti F, Pertici I, et al. CTGF/CCN2 exerts profibrotic action in myoblasts via the up-regulation of sphingosine kinase-1/S1P3 signaling axis: Implications in the action mechanism of TGFβ. Biochim Biophys Acta. 2015,1851(2):194-202. doi: 10.1016/j.bbalip.2014.11.011
    Read this paper
  • Donati C, Marseglia G, Magi A, et al. Sphingosine 1-phosphate induces differentiation of mesoangioblasts towards smooth muscle. A role for GATA6. PLoS One. 2011,6(5):e20389. doi: 10.1371/journal.pone.0020389
    Read this paper
  • Cencetti F, Bernacchioni C, Nincheri P, et al. Transforming growth factor-beta1 induces transdifferentiation of myoblasts into myofibroblasts via up-regulation of sphingosine kinase-1/S1P3 axis. Mol Biol Cell. 2010,21(6):1111-24.
    Read this paper
  • Nincheri P, Luciani P, Squecco R, et al. Sphingosine 1-phosphate induces differentiation of adipose tissue-derived mesenchymal stem cells towards smooth muscle cells. Cell Mol Life Sci. 2009,66(10):1741-54. doi: 10.1007/s00018-009-9181-8
    Read this paper

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Figure 1. Western blot analysis of TAGLN/Transgelin using anti-TAGLN/Transgelin antibody (A03962-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: rat stomach tissue lysates, Lane 3: mouse spleen tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAGLN/Transgelin antigen affinity purified polyclonal antibody (Catalog # A03962-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TAGLN/Transgelin at approximately 22KD. The expected band size for TAGLN/Transgelin is at 22KD.
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