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Goat anti-SOCS1

EB05040
Everest Biotech
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetSOCS1
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-SOCS1 Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Immunofluorescence: Expression of the protein seen in the cytoplasm and nucleus of MCF7 cells. Recommended concentration: 10microg/ml. Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml.
  • Applications
    Flow Cytometry, ImmunoFluorescence, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, FC, IF, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID8651
  • Target name
    SOCS1
  • Target description
    suppressor of cytokine signaling 1
  • Target synonyms
    AISIMD; CIS1; CISH1; cytokine-inducible SH2 protein 1; JAB; JAK binding protein; SOCS-1; SSI1; SSI-1; STAT induced SH3 protein 1; STAT-induced STAT inhibitor 1; suppressor of cytokine signaling 1; Tec-interacting protein 3; TIP3; TIP-3
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_003736.1. GeneIDs all Nonhuman: 12703 (mouse);. Purification: Antigen affinity purified. Names and symbols: SOCS1; JAB; CIS1; SSI1; TIP3; CISH1; Cish1; SSI-1; SOCS-1; suppressor of cytokine signaling 1; JAK binding protein; Tec-interacting protein 3; STAT induced SH3 protein 1; cytokine-inducible SH2 protein 1
  • Reactivity Supplier
    Human, Mouse, Dog, Cow, Rat, Pig
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SOCS1 using anti-SOCS1 antibody (A00448). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOCS1 antigen affinity purified polyclonal antibody (Catalog # A00448) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOCS1 at approximately 27 kDa. The expected band size for SOCS1 is at 24 kDa.
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