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ProductsBack/Goat anti-SORBS1 / ponsin (internal)

Goat anti-SORBS1 / ponsin (internal)

EB07177
Everest Biotech
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetSORBS1
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-SORBS1 / ponsin (internal) Antibody
  • Delivery Days Customer
    5
  • Applications
    ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID10580
  • Target name
    SORBS1
  • Target description
    sorbin and SH3 domain containing 1
  • Target synonyms
    CAP; c-Cbl associated protein; Fas-ligand associated factor 2; FLAF2; ponsin; R85FL; SH3 domain protein 5; SH3D5; SH3P12; SORB1; sorbin and SH3 domain-containing protein 1
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_006425.2; NP_056200.1; NP_001030126.1; NP_001030127.1; NP_001030128.1; NP_079267.1; NP_001030129.1. Purification: Antigen affinity purified. Names and symbols: SORBS1; sorbin and SH3 domain containing 1 ; CAP; DKFZp451C066; DKFZp586P1422; FLAF2; FLJ12406; KIAA1296; R85FL; SH3D5; SH3P12; SORB1 ; Fas-ligand associated factor 2; SH3-domain protein 5; SH3-domain protein 5 (ponsin); c-Cbl associated protein; ponsin
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SORBS1 using anti-SORBS1 antibody (A04426-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SORBS1 antigen affinity purified polyclonal antibody (Catalog # A04426-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SORBS1 at approximately 160KD. The expected band size for SORBS1 is at 142KD.
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