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Goat anti-Triosephosphate isomerase Antibody

EB06643
Everest Biotech
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityBovine, Canine, Human, Mouse, Rat
TargetTPI1
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-Triosephosphate isomerase Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.
  • Applications
    Flow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, WB, IHC, FC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID7167
  • Target name
    TPI1
  • Target description
    triosephosphate isomerase 1
  • Target synonyms
    HEL-S-49, TIM, TPI, TPID, triosephosphate isomerase, epididymis secretory protein Li 49, methylglyoxal synthase, triose-phosphate isomerase
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_000356.1; NP_001152759.1; NP_001244955.1. GeneIDs all Nonhuman: 21991 (mouse); 24849 (rat);. Purification: Antigen affinity purified. Names and symbols: TPI1; triosephosphate isomerase 1; TPI; MGC88108
  • Reactivity
    Bovine, Canine, Human, Mouse, Rat
  • Reactivity Supplier
    Human, Mouse, Rat, Dog, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Honoré B, Buus S, Claësson MH. Identification of differentially expressed proteins in spontaneous thymic lymphomas from knockout mice with deletion of p53. Proteome Sci. 2008,6:18. doi: 10.1186/1477-5956-6-18
    Read this paper
  • Vorum H, Østergaard M, Rice GE, et al. Identification of differentially regulated proteins in a patient with Leber's Congenital Amaurosis--a proteomic study. Proteome Sci. 2007,5:5.
    Read this paper

FAQ

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Figure 1. Western blot analysis of TPI1 using anti-TPI1 antibody (A02559-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: rat kidney tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TPI1 antigen affinity purified polyclonal antibody (Catalog # A02559-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TPI1 at approximately 27 kDa. The expected band size for TPI1 is at 27 kDa.
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