![Wild-type (WT) and GRK2 knockout (KO) 293T cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 antibody [HL1562] (GTX637038) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Wild-type (WT) and GRK2 knockout (KO) 293T cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 antibody [HL1562] (GTX637038) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637038/GTX637038_T-44704_20220624_WB_KO_watermerk_22062919_833.webp)
Wild-type (WT) and GRK2 knockout (KO) 293T cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 antibody [HL1562] (GTX637038) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GRK2 antibody [HL1562]
GTX637038
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetGRK2
Overview
- SupplierGeneTex
- Product NameGRK2 antibody [HL1562]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL1562
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID156
- Target nameGRK2
- Target descriptionG protein-coupled receptor kinase 2
- Target synonymsADRBK1, BARK1, BETA-ARK1, beta-adrenergic receptor kinase 1, adrenergic beta receptor kinase 1, beta-ARK-1
- HostRabbit
- IsotypeIgG
- Protein IDP25098
- Protein NameBeta-adrenergic receptor kinase 1
- Scientific DescriptionThis gene encodes a member of the G protein-coupled receptor kinase family of proteins. The encoded protein phosphorylates the beta-adrenergic receptor as well as a wide range of other substrates including non-GPCR cell surface receptors, and cytoskeletal, mitochondrial, and transcription factor proteins. Data from rodent models supports a role for this gene in embryonic development, heart function and metabolism. Elevated expression of this gene has been observed in human patients with heart failure and Alzheimers disease. [provided by RefSeq, Sep 2017]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
![GRK2 antibody [HL1562] detects GRK2 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse lymph node. GRK2 stained by GRK2 antibody [HL1562] (GTX637038) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min GRK2 antibody [HL1562] detects GRK2 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse lymph node. GRK2 stained by GRK2 antibody [HL1562] (GTX637038) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX637038/GTX637038_T-44704_20220701_IHC-P_M_22070501_892.webp)
![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 antibody [HL1562] (GTX637038) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 antibody [HL1562] (GTX637038) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637038/GTX637038_44767_20220812_WB_22081423_564.webp)
![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 antibody [HL1562] (GTX637038) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 antibody [HL1562] (GTX637038) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637038/GTX637038_44767_20220812_WB_B_22081423_103.webp)
![Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 antibody [HL1562] (GTX637038) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 antibody [HL1562] (GTX637038) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637038/GTX637038_T-44704_20220819_WB_M_tissue_22082402_129.webp)
![GRK2 antibody [HL1562] detects GRK2 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. GRK2 stained by GRK2 antibody [HL1562] (GTX637038) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min GRK2 antibody [HL1562] detects GRK2 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. GRK2 stained by GRK2 antibody [HL1562] (GTX637038) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX637038/GTX637038_44767_20221227_IHC-P_multiple_R_22122821_919.webp)
![GRK2 antibody [HL1562] detects GRK2 protein by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 10 min. Green: GRK2 stained by GRK2 antibody [HL1562] (GTX637038) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). Scale bar= 10μm. GRK2 antibody [HL1562] detects GRK2 protein by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 10 min. Green: GRK2 stained by GRK2 antibody [HL1562] (GTX637038) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). Scale bar= 10μm.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637038/GTX637038_44767_20221230_ICC_IF_22122901_202.webp)



![Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with GRK2 antibody [C2C3], C-term (GTX101682) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX101682/GTX101682_43691_20211119_WB_M_w_23060100_190.webp)

![ELISA analysis of antigen using GTX60430 GRK2 antibody [3F8].
Red : Control antigen 100ng
Purple : Antigen 10ng
Green : Antigen 50ng
Blue : Antigen 100ng](https://www.genetex.com/upload/website/prouct_img/normal/GTX60430/GTX60430_20170912_ELISA_w_23061123_556.webp)
![Various tissue extracts (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 (phospho Ser670) antibody [HL1035] (GTX635875) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX635875/GTX635875_44200_20220701_WB_M_thymus_22070501_687.webp)
