IHC-P analysis of mouse breast tissue using GTX22791 GRP94 antibody [9G10]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200
![IHC-P analysis of mouse liver tissue using GTX22791 GRP94 antibody [9G10]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX22791/GTX22791_1117_IHC-P_w_23060620_387.webp "IHC-P analysis of mouse liver tissue using GTX22791 GRP94 antibody [9G10]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200")
![IHC-P analysis of mouse lymph node tissue using GTX22791 GRP94 antibody [9G10]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX22791/GTX22791_1118_IHC-P_w_23060620_401.webp "IHC-P analysis of mouse lymph node tissue using GTX22791 GRP94 antibody [9G10]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200")
![IHC-P analysis of poorly differentiated adenocarcinoma tissue from human colon tissue sections using GTX22791 GRP94 antibody [9G10]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0) buffer for 20 minutes at 95oC Dilution : 1:100](https://www.genetex.com/upload/website/prouct_img/normal/GTX22791/GTX22791_1119_IHC-P_w_23060620_840.webp "IHC-P analysis of poorly differentiated adenocarcinoma tissue from human colon tissue sections using GTX22791 GRP94 antibody [9G10]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0) buffer for 20 minutes at 95oC Dilution : 1:100")
![WB analysis of 2-fold serial dilutions of HeLa cell lysate(starting at 10 ug, per well) using GTX22791 GRP94 antibody [9G10]. Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX22791/GTX22791_1595_WB_w_23060620_858.webp "WB analysis of 2-fold serial dilutions of HeLa cell lysate(starting at 10 ug, per well) using GTX22791 GRP94 antibody [9G10]. Dilution : 1:1000")
![WB analysis of 25 ug of Hela (lane 1), C6 (lane 2) and NIH-3T3 (lane 3) lysates using GTX22791 GRP94 antibody [9G10]. Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX22791/GTX22791_1596_WB_w_23060620_938.webp "WB analysis of 25 ug of Hela (lane 1), C6 (lane 2) and NIH-3T3 (lane 3) lysates using GTX22791 GRP94 antibody [9G10]. Dilution : 1:200")
![ICC/IF analysis of U2OS cells using GTX22791 GRP94 antibody [9G10]. Fixation : 4% paraformaldehyde Permeabilization : 0.1% Triton X-100 in PBS for 15 minutes Dilution : 1:100 for at least 1 hour at room temperature](https://www.genetex.com/upload/website/prouct_img/normal/GTX22791/GTX22791_451_ICC-IF_w_23060620_964.webp "ICC/IF analysis of U2OS cells using GTX22791 GRP94 antibody [9G10]. Fixation : 4% paraformaldehyde Permeabilization : 0.1% Triton X-100 in PBS for 15 minutes Dilution : 1:100 for at least 1 hour at room temperature")
![ICC/IF analysis of C6 cells using GTX22791 GRP94 antibody [9G10]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:100 overnight at 4 oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX22791/GTX22791_452_ICC-IF_w_23060620_104.webp "ICC/IF analysis of C6 cells using GTX22791 GRP94 antibody [9G10]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:100 overnight at 4 oC")
IHC-P analysis of mouse breast tissue using GTX22791 GRP94 antibody [9G10]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200
GRP94 antibody [9G10]
GTX22791
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Chicken, Hamster, Human, Mouse, Porcine, Rat, Xenopus
TargetHSP90B1
Overview
- SupplierGeneTex
- Product NameGRP94 antibody [9G10]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:100-1:1000. ICC/IF: 1:250. IHC-P: 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID9G10
- ConjugateUnconjugated
- Gene ID7184
- Target nameHSP90B1
- Target descriptionheat shock protein 90 beta family member 1
- Target synonyms94 kDa glucose-regulated protein; ECGP; endoplasmin; endothelial cell (HBMEC) glycoprotein; epididymis luminal protein 35; epididymis secretory sperm binding protein Li 125m; GP96; GRP94; heat shock protein 90 kDa beta member 1; heat shock protein 90kDa beta (Grp94), member 1; heat shock protein 90kDa beta family member 1; HEL35; HEL-S-125m; stress-inducible tumor rejection antigen gp96; TRA1; tumor rejection antigen (gp96) 1; tumor rejection antigen 1
- HostRat
- IsotypeIgG2a
- Protein IDP14625
- Protein NameEndoplasmin
- Scientific DescriptionThis gene encodes a member of a family of adenosine triphosphate(ATP)-metabolizing molecular chaperones with roles in stabilizing and folding other proteins. The encoded protein is localized to melanosomes and the endoplasmic reticulum. Expression of this protein is associated with a variety of pathogenic states, including tumor formation. There is a microRNA gene located within the 5 exon of this gene. There are pseudogenes for this gene on chromosomes 1 and 15. [provided by RefSeq, Aug 2012]
- ReactivityBovine, Chicken, Hamster, Human, Mouse, Porcine, Rat, Xenopus
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203