![IHC-P analysis of mouse breast tissue using GTX22791 GRP94 antibody [9G10]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200 IHC-P analysis of mouse breast tissue using GTX22791 GRP94 antibody [9G10]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX22791/GTX22791_1116_IHC-P_w_23060620_550.webp)
IHC-P analysis of mouse breast tissue using GTX22791 GRP94 antibody [9G10]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200
GRP94 antibody [9G10]
GTX22791
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetHSP90B1
Overview
- SupplierGeneTex
- Product NameGRP94 antibody [9G10]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:100-1:1000. ICC/IF: 1:250. IHC-P: 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID9G10
- ConjugateUnconjugated
- Gene ID7184
- Target nameHSP90B1
- Target descriptionheat shock protein 90 beta family member 1
- Target synonymsECGP, GP96, GRP94, HEL-S-125m, HEL35, TRA1, endoplasmin, 94 kDa glucose-regulated protein, endothelial cell (HBMEC) glycoprotein, epididymis luminal protein 35, epididymis secretory sperm binding protein Li 125m, heat shock protein 90 kDa beta member 1, heat shock protein 90kDa beta (Grp94), member 1, heat shock protein 90kDa beta family member 1, heat shock protein family C member 4, stress-inducible tumor rejection antigen gp96, tumor rejection antigen (gp96) 1, tumor rejection antigen 1
- HostRat
- IsotypeIgG2a
- Protein IDP14625
- Protein NameEndoplasmin
- Scientific DescriptionThis gene encodes a member of a family of adenosine triphosphate(ATP)-metabolizing molecular chaperones with roles in stabilizing and folding other proteins. The encoded protein is localized to melanosomes and the endoplasmic reticulum. Expression of this protein is associated with a variety of pathogenic states, including tumor formation. There is a microRNA gene located within the 5 exon of this gene. There are pseudogenes for this gene on chromosomes 1 and 15. [provided by RefSeq, Aug 2012]
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
![IHC-P analysis of mouse liver tissue using GTX22791 GRP94 antibody [9G10]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200 IHC-P analysis of mouse liver tissue using GTX22791 GRP94 antibody [9G10]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX22791/GTX22791_1117_IHC-P_w_23060620_387.webp)
![IHC-P analysis of mouse lymph node tissue using GTX22791 GRP94 antibody [9G10]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200 IHC-P analysis of mouse lymph node tissue using GTX22791 GRP94 antibody [9G10]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX22791/GTX22791_1118_IHC-P_w_23060620_401.webp)
![IHC-P analysis of poorly differentiated adenocarcinoma tissue from human colon tissue sections using GTX22791 GRP94 antibody [9G10]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0) buffer for 20 minutes at 95oC Dilution : 1:100 IHC-P analysis of poorly differentiated adenocarcinoma tissue from human colon tissue sections using GTX22791 GRP94 antibody [9G10]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0) buffer for 20 minutes at 95oC Dilution : 1:100](https://www.genetex.com/upload/website/prouct_img/normal/GTX22791/GTX22791_1119_IHC-P_w_23060620_840.webp)
![WB analysis of 2-fold serial dilutions of HeLa cell lysate(starting at 10 ug, per well) using GTX22791 GRP94 antibody [9G10]. Dilution : 1:1000 WB analysis of 2-fold serial dilutions of HeLa cell lysate(starting at 10 ug, per well) using GTX22791 GRP94 antibody [9G10]. Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX22791/GTX22791_1595_WB_w_23060620_858.webp)
![WB analysis of 25 ug of Hela (lane 1), C6 (lane 2) and NIH-3T3 (lane 3) lysates using GTX22791 GRP94 antibody [9G10]. Dilution : 1:200 WB analysis of 25 ug of Hela (lane 1), C6 (lane 2) and NIH-3T3 (lane 3) lysates using GTX22791 GRP94 antibody [9G10]. Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX22791/GTX22791_1596_WB_w_23060620_938.webp)
![ICC/IF analysis of U2OS cells using GTX22791 GRP94 antibody [9G10]. Fixation : 4% paraformaldehyde Permeabilization : 0.1% Triton X-100 in PBS for 15 minutes Dilution : 1:100 for at least 1 hour at room temperature ICC/IF analysis of U2OS cells using GTX22791 GRP94 antibody [9G10]. Fixation : 4% paraformaldehyde Permeabilization : 0.1% Triton X-100 in PBS for 15 minutes Dilution : 1:100 for at least 1 hour at room temperature](https://www.genetex.com/upload/website/prouct_img/normal/GTX22791/GTX22791_451_ICC-IF_w_23060620_964.webp)
![ICC/IF analysis of C6 cells using GTX22791 GRP94 antibody [9G10]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:100 overnight at 4 oC ICC/IF analysis of C6 cells using GTX22791 GRP94 antibody [9G10]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:100 overnight at 4 oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX22791/GTX22791_452_ICC-IF_w_23060620_104.webp)


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