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ProductsBack/GSTM1 Mouse Monoclonal Antibody [Clone ID: 1H4A4]
Western blot analysis using GSTM1 mAb against human GSTM1 (AA: 23-181) recombinant protein. (Expected MW is 25.7 kDa)
Western blot analysis using GSTM1 mAb against human GSTM1 (AA: 23-181) recombinant protein. (Expected MW is 25.7 kDa)
Western blot analysis using GSTM1 mAb against human GSTM1 (AA: 23-181) recombinant protein. (Expected MW is 25.7 kDa)

GSTM1 Mouse Monoclonal Antibody [Clone ID: 1H4A4]

AM06705SU-N
OriGene
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Monkey
TargetGSTM1
Price on request
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    GSTM1 Mouse Monoclonal Antibody [Clone ID: 1H4A4]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    1H4A4
  • Gene ID2944
  • Target name
    GSTM1
  • Target description
    glutathione S-transferase mu 1
  • Target synonyms
    GST1, GSTM1-1, GSTM1a-1a, GSTM1b-1b, GTH4, GTM1, H-B, MU, MU-1, glutathione S-transferase Mu 1, GST HB subunit 4, GST class-mu 1, HB subunit 4, S-(hydroxyalkyl)glutathione lyase, glutathione S-alkyltransferase, glutathione S-aralkyltransferase, glutathione S-aryltransferase, glutathione S-transferase M1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP09488
  • Protein Name
    Glutathione S-transferase Mu 1
  • Scientific Description
    GSTM1 mouse monoclonal antibody, clone 1H4A4, Ascites
  • Reactivity
    Human, Monkey
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of GSTM1 using anti-GSTM1 antibody (A00569-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat lung tissue lysates, Lane 3: mouse stomach tissue lysates, Lane 4: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GSTM1 antigen affinity purified polyclonal antibody (Catalog # A00569-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GSTM1 at approximately 26KD. The expected band size for GSTM1 is at 26KD.
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