Wild-type (WT) and HDAC1 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody [HL1691] (GTX637290) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![HDAC1 antibody [HL1691] detects HDAC1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HDAC1 stained by HDAC1 antibody [HL1691] (GTX637290) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637290/GTX637290_T-44767_20220916_ICC_IF_22102723_828.webp "HDAC1 antibody [HL1691] detects HDAC1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HDAC1 stained by HDAC1 antibody [HL1691] (GTX637290) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.")
![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody [HL1691] (GTX637290) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637290/GTX637290_44844_20221028_WB_22110201_167.webp "Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody [HL1691] (GTX637290) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody [HL1691] (GTX637290) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637290/GTX637290_44844_20230707_WB_multiple_B_23071223_639.webp "Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody [HL1691] (GTX637290) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
Wild-type (WT) and HDAC1 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody [HL1691] (GTX637290) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
HDAC1 antibody [HL1691]
GTX637290
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetHDAC1
Overview
- SupplierGeneTex
- Product NameHDAC1 antibody [HL1691]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL1691
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID3065
- Target nameHDAC1
- Target descriptionhistone deacetylase 1
- Target synonymsGON-10, HD1, KDAC1, RPD3, RPD3L1, histone deacetylase 1, protein deacetylase HDAC1, protein deacylase HDAC1, protein decrotonylase HDAC1, reduced potassium dependency, yeast homolog-like 1
- HostRabbit
- IsotypeIgG
- Protein IDQ13547
- Protein NameHistone deacetylase 1
- Scientific DescriptionHistone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis. [provided by RefSeq, Jul 2008]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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