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ProductsBack/HDAC1 Recombinant Rabbit mAb, Nuclear Loading Control

HDAC1 Recombinant Rabbit mAb, Nuclear Loading Control

ORB559205
Biorbyt
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetHDAC1
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Overview

  • Supplier
    Biorbyt
  • Product Name
    HDAC1 Recombinant Rabbit mAb, Nuclear Loading Control
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:50-200, ICC/IF=1:50-200, IF=1:50-200 ICC, IF, IHC-Fr, IHC-P, WB
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    3C3
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3065
  • Target name
    HDAC1
  • Target description
    histone deacetylase 1
  • Target synonyms
    GON-10, HD1, KDAC1, RPD3, RPD3L1, histone deacetylase 1, protein deacetylase HDAC1, protein deacylase HDAC1, protein decrotonylase HDAC1, reduced potassium dependency, yeast homolog-like 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13547
  • Protein Name
    Histone deacetylase 1
  • Scientific Description
    HDAC1 Recombinant Rabbit mAb, Nuclear Loading Control
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of HDAC1 using anti-HDAC1 antibody (A00256-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HDAC1 antigen affinity purified polyclonal antibody (Catalog # A00256-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HDAC1 at approximately 65 kDa. The expected band size for HDAC1 is at 65 kDa.
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