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ProductsBack/HDAC1 Rabbit pAb, Nuclear Loading Control

HDAC1 Rabbit pAb, Nuclear Loading Control

ORB704381
Biorbyt
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetHDAC1
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Overview

  • Supplier
    Biorbyt
  • Product Name
    HDAC1 Rabbit pAb, Nuclear Loading Control
  • Delivery Days Customer
    10
  • Applications
    Western Blot
  • Applications Supplier
    WB=1:500-2000 WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3065
  • Target name
    HDAC1
  • Target description
    histone deacetylase 1
  • Target synonyms
    GON-10, HD1, KDAC1, RPD3, RPD3L1, histone deacetylase 1, protein deacetylase HDAC1, protein deacylase HDAC1, protein decrotonylase HDAC1, reduced potassium dependency, yeast homolog-like 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13547
  • Protein Name
    Histone deacetylase 1
  • Scientific Description
    HDAC1 Rabbit pAb, Nuclear Loading Control
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of HDAC1 using anti-HDAC1 antibody (A00256-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HDAC1 antigen affinity purified polyclonal antibody (Catalog # A00256-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HDAC1 at approximately 65 kDa. The expected band size for HDAC1 is at 65 kDa.
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