More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/HDAC3 antibody
Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody (GTX113303) diluted at 1:500.
Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody (GTX113303) diluted at 1:500.
Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody (GTX113303) diluted at 1:500.

HDAC3 antibody

GTX113303
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat, Zebra Fish
TargetHDAC3
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    GeneTex
  • Product Name
    HDAC3 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.32 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID8841
  • Target name
    HDAC3
  • Target description
    histone deacetylase 3
  • Target synonyms
    HD3, KDAC3, RPD3, RPD3-2, histone deacetylase 3, SMAP45, protein deacetylase HDAC3, protein deacylase HDAC3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO15379
  • Protein Name
    Histone deacetylase 3
  • Scientific Description
    Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene. [provided by RefSeq]
  • Reactivity
    Human, Mouse, Rat, Zebra Fish
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Martirosian V, Deshpande K, Zhou H, et al. Medulloblastoma uses GABA transaminase to survive in the cerebrospinal fluid microenvironment and promote leptomeningeal dissemination. Cell Rep. 2021,35(13):109302. doi: 10.1016/j.celrep.2021.109302
    Read this paper
  • Miscianinov V, Martello A, Rose L, et al. MicroRNA-148b Targets the TGF-β Pathway to Regulate Angiogenesis and Endothelial-to-Mesenchymal Transition during Skin Wound Healing. Mol Ther. 2018,26(8):1996-2007. doi: 10.1016/j.ymthe.2018.05.002
    Read this paper
  • Armour SM, Remsberg JR, Damle M, et al. An HDAC3-PROX1 corepressor module acts on HNF4α to control hepatic triglycerides. Nat Commun. 2017,8(1):549. doi: 10.1038/s41467-017-00772-5
    Read this paper
  • Emmett MJ, Lim HW, Jager J, et al. Histone deacetylase 3 prepares brown adipose tissue for acute thermogenic challenge. Nature. 2017,546(7659):544-548. doi: 10.1038/nature22819
    Read this paper
  • Soriano FX, Hardingham GE. In cortical neurons HDAC3 activity suppresses RD4-dependent SMRT export. PLoS One. 2011,6(6):e21056. doi: 10.1371/journal.pone.0021056
    Read this paper

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

Anti-HDAC3 Monoclonal Antibody
Product group Antibodies
BPS Bioscience
Anti-HDAC3 Monoclonal Antibody25289
ApplicationsImmunoFluorescence, ChIP Chromatin ImmunoPrecipitation
ReactivityHuman
TargetHDAC3
  • SizePrice
Product group Antibodies
RayBiotech
Anti-HDAC3 Antibody144-02139
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetHDAC3
  • SizePrice
Figure 1. Western blot analysis of HDAC3 using anti-HDAC3 antibody (A00839). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human MOLT-4 whole cell lysates, Lane 5: human MCF-7 whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: human CACO-2 whole cell lysates, Lane 8: rat thymus tissue lysates, Lane 9: rat C6 whole cell lysates, Lane 10: mouse ANA-1 whole cell lysates, Lane 11: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HDAC3 antigen affinity purified polyclonal antibody (Catalog # A00839) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HDAC3 at approximately 49 kDa. The expected band size for HDAC3 is at 49 kDa.
Product group Antibodies
Boster Bio
Anti-HDAC3 Antibody Picoband(r)A00839-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetHDAC3
  • SizePrice
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody [GT1144] (GTX00834) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Product group Antibodies
GeneTex
HDAC3 antibody [GT1144]GTX00834
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetHDAC3
  • SizePrice
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody [C3], C-term (GTX109679) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Product group Antibodies
References
GeneTex
HDAC3 antibody [C3], C-termGTX109679
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityDrosophila, Human, Mouse, Rat
TargetHDAC3
  • SizePrice
ICC/IF analysis of 4% paraformaldehyde fixed HeLa cells using GTX60366 HDAC3 antibody [GT9007]. Red : Primary antibody Blue : DAPI Dilution : 1:500
Product group Antibodies
GeneTex
HDAC3 antibody [GT9007]GTX60366
ApplicationsImmunoFluorescence, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry
ReactivityHuman
TargetHDAC3
  • SizePrice
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody [HL1749] (GTX637399) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Product group Antibodies
GeneTex
HDAC3 antibody [HL1749]GTX637399
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityDrosophila, Human, Mouse, Rat
TargetHDAC3
  • SizePrice
Product group Antibodies
Biomatik
HDAC3 Polyclonal AntibodyCAC13761
ApplicationsImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoHistoChemistry
ReactivityMouse
TargetHDAC3
  • SizePrice
Product group Antibodies
Bioss
HDAC3 Recombinant Antbody Kit (KD-Validated)BSM-90299R
ApplicationsFlow Cytometry, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetHDAC3
  • SizePrice
Product group Antibodies
Assay Genie
HDAC3 AntibodyCAB2139
ReactivityHuman
TargetHDAC3
  • SizePrice