HDAC7 antibody immunoprecipitates HDAC7 protein in IP experiments. IP Sample: 1000 μg 293T whole cell lysate/extract A. 50 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of HDAC7 protein by 2 μg of HDAC7 antibody (GTX114179) 10% SDS-PAGE The immunoprecipitated HDAC7 protein was detected by HDAC7 antibody (GTX114179) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
![Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HDAC7 antibody [C2C3], C-term (GTX114179) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX114179/GTX114179_40135_20190614_WB_w_23060501_673.webp "Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HDAC7 antibody [C2C3], C-term (GTX114179) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
HDAC7 antibody immunoprecipitates HDAC7 protein in IP experiments. IP Sample: 1000 μg 293T whole cell lysate/extract A. 50 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of HDAC7 protein by 2 μg of HDAC7 antibody (GTX114179) 10% SDS-PAGE The immunoprecipitated HDAC7 protein was detected by HDAC7 antibody (GTX114179) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
HDAC7 antibody [C2C3], C-term
GTX114179
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetHDAC7
Overview
- SupplierGeneTex
- Product NameHDAC7 antibody [C2C3], C-term
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:500-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1.75 mg/ml
- ConjugateUnconjugated
- Gene ID51564
- Target nameHDAC7
- Target descriptionhistone deacetylase 7
- Target synonymsHD7, HD7A, HDAC7A, histone deacetylase 7, histone deacetylase 7A, protein deacetylase HDAC7
- HostRabbit
- IsotypeIgG
- Protein IDQ8WUI4
- Protein NameHistone deacetylase 7
- Scientific DescriptionHistones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene has sequence homology to members of the histone deacetylase family. This gene is orthologous to mouse HDAC7 gene whose protein promotes repression mediated via the transcriptional corepressor SMRT. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
Datasheet
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