IP analysis of HEK293T cells expressing DDDDK-tagged human HDAC7. The precipitated hHDAC7 protein was detected using anti-DDDDK Tag antibody. Lane 1: IP from 180 microg of 293T cell extract (expressing DDDDK tagged human HDAC7) using 1.8 microg HDAC7 antibody(GTX12174) Lane 2: Negative control (extract without antibody).
IP analysis of HEK293T cells expressing DDDDK-tagged human HDAC7. The precipitated hHDAC7 protein was detected using anti-DDDDK Tag antibody. Lane 1: IP from 180 microg of 293T cell extract (expressing DDDDK tagged human HDAC7) using 1.8 microg HDAC7 antibody(GTX12174) Lane 2: Negative control (extract without antibody).
HDAC7 antibody
GTX12174
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetHDAC7
Overview
- SupplierGeneTex
- Product NameHDAC7 antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1,000. ICC/IF: 1:50. IP: 1.0-1.5 microg. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- ConjugateUnconjugated
- Gene ID51564
- Target nameHDAC7
- Target descriptionhistone deacetylase 7
- Target synonymsHD7; HD7A; HDAC7A; histone deacetylase 7; histone deacetylase 7A
- HostRabbit
- IsotypeIgG
- Protein IDQ8WUI4
- Protein NameHistone deacetylase 7
- Scientific DescriptionHDAC7 is a member of the class II mammalian histone deacetylases, which plays an important role in modulating the eukaryotic chromatin structure. Human HDAC7 is composed of 912 amino acid residues. Although HDAC7 is localized mostly to the cell nucleus, it is also found in the cytoplasm, suggesting nucleo-cytoplasmic shuttling. The histone deacetylase activity of HDAC7 maps to a carboxy-terminal domain and is dependent on interaction with class I HDACs in the nucleus. It is an active component of different transcriptional corepressor complexes that can be recruited to specific promoter regions via interactions with a growing number of sequence specific transcriptional factors. HDAC7 catalyzes removal of acetyl-groups from acetyl-lysines of histones and promotes compaction of chromatin in these regions, leading to the inhibition of gene transcription.
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203