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HDAC7 Antibody Pair

CSB-EAP17899
Cusabio
ApplicationsELISA
Product group Antibodies
ReactivityBovine, Canine, Goat, Human, Porcine, Sheep
TargetHDAC7
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Overview

  • Supplier
    Cusabio
  • Product Name
    HDAC7 Antibody Pair
  • Delivery Days Customer
    9
  • Application Supplier Note
    We recommend using the capture antibody at a concentration of 0.5ug/ml and the detection antibody at a concentration of 0.34ug/ml.Optimal dilutions should be determined experimentally by the researcher.
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Conjugate
    Biotin
  • Gene ID51564
  • Target name
    HDAC7
  • Target description
    histone deacetylase 7
  • Target synonyms
    HD7; HD7A; HDAC7A; histone deacetylase 7; histone deacetylase 7A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8WUI4
  • Protein Name
    Histone deacetylase 7
  • Reactivity
    Bovine, Canine, Goat, Human, Porcine, Sheep
  • UNSPSC
    41116163

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Figure 1. Western blot analysis of HDAC7 using anti-HDAC7 antibody (PB9629). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Spleen Tissue Lysate at 50ug, Lane 2: Rat Thymus Tissue Lysate at 50ug, Lane 3: Human Placenta Tissue Lysate at 50ug, Lane 4: HELA Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HDAC7 antigen affinity purified polyclonal antibody (Catalog # PB9629) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HDAC7 at approximately 103 kDa. The expected band size for HDAC7 is at 103 kDa.
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