![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody [GT17811] (GTX633677) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody [GT17811] (GTX633677) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX633677/GTX633677_42590_20230519_WB_multiple_B_23053001_670.webp)
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody [GT17811] (GTX633677) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Heme Oxygenase 1 antibody [GT17811]
GTX633677
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetHMOX1
Overview
- SupplierGeneTex
- Product NameHeme Oxygenase 1 antibody [GT17811]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT17811
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID3162
- Target nameHMOX1
- Target descriptionheme oxygenase 1
- Target synonymsHMOX1D, HO-1, HSP32, bK286B10, heme oxygenase 1, heat shock protein, 32-kD, heme oxygenase (decycling) 1
- HostMouse
- IsotypeIgG2a
- Protein IDP09601
- Protein NameHeme oxygenase 1
- Scientific DescriptionHeme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family. [provided by RefSeq]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
![Heme Oxygenase 1 antibody [GT17811] detects Heme Oxygenase 1 protein at endoplasmic reticulum by immunofluorescent analysis. Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Heme Oxygenase 1 stained by Heme Oxygenase 1 antibody [GT17811] (GTX633677) diluted at 1:2000. Blue: Hoechst 33342 staining. Scale bar= 10μm. Heme Oxygenase 1 antibody [GT17811] detects Heme Oxygenase 1 protein at endoplasmic reticulum by immunofluorescent analysis. Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Heme Oxygenase 1 stained by Heme Oxygenase 1 antibody [GT17811] (GTX633677) diluted at 1:2000. Blue: Hoechst 33342 staining. Scale bar= 10μm.](https://www.genetex.com/upload/website/prouct_img/normal/GTX633677/GTX633677_42590_20180718_ICC_IF_CoCl2_w_23061202_299.webp)
![Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody [GT17811] (GTX633677) diluted at 1:1000. Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody [GT17811] (GTX633677) diluted at 1:1000.](https://www.genetex.com/upload/website/prouct_img/normal/GTX633677/GTX633677_42590_20161013_WB_CoCl2_w_23061202_651.webp)

![Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody [GT17811] (GTX633677) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody [GT17811] (GTX633677) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](https://www.genetex.com/upload/website/prouct_img/normal/GTX633677/GTX633677_42590_20231124_WB_TPM_watermark_23112922_425.webp)




![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody [GT1334] (GTX633676) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX633676/GTX633676_42590_20230519_WB_multiple_B_23053001_657.webp)
![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody [GT664] (GTX633693) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX633693/GTX633693_42639_20230519_WB_multiple_B_23053001_417.webp)
![Wild-type (WT) and HMOX1 knockout (KO) HeLa cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody [HL1780] (GTX637432) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637432/GTX637432_T-44802_20221209_WB_KO_watermark_22121123_519.webp)