More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/HES1 antibody [HL2198]
MCF-7 whole cell and nuclear extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HES1 antibody [HL2198] (GTX638202) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
MCF-7 whole cell and nuclear extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HES1 antibody [HL2198] (GTX638202) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
MCF-7 whole cell and nuclear extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HES1 antibody [HL2198] (GTX638202) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

HES1 antibody [HL2198]

GTX638202
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetHES1
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    GeneTex
  • Product Name
    HES1 antibody [HL2198]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1000-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    HL2198
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3280
  • Target name
    HES1
  • Target description
    hes family bHLH transcription factor 1
  • Target synonyms
    HES-1, HHL, HRY, bHLHb39, transcription factor HES-1, class B basic helix-loop-helix protein 39, hairy homolog, hairy-like protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14469
  • Protein Name
    Transcription factor HES-1
  • Scientific Description
    This protein belongs to the basic helix-loop-helix family of transcription factors. It is a transcriptional repressor of genes that require a bHLH protein for their transcription. The protein has a particular type of basic domain that contains a helix interrupting protein that binds to the N-box rather than the canonical E-box. [provided by RefSeq, Jul 2008]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
Assay Genie
HES1 Monoclonal AntibodyCAB0925
ReactivityMouse
TargetHES1
  • SizePrice
Immunohistochemistry of paraffin-embedded human thyroid cancer using CSB-PA245265 at dilution of 1:20
Product group Antibodies
Cusabio
HES1 AntibodyCSB-PA245265
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetHES1
  • SizePrice
Anti-HES1 Antibody
Product group Antibodies
Antibodies.com
Anti-HES1 AntibodyA43945
ApplicationsWestern Blot
ReactivityHuman
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
HES1 / HES-1 AntibodyLS-C747087
ApplicationsWestern Blot
ReactivityHuman, Rat
TargetHES1
  • SizePrice
Figure 1. Western blot analysis of Hes1 using anti-Hes1 antibody (M01459). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hes1 antigen affinity purified monoclonal antibody (Catalog # M01459) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Hes1 at approximately 30 kDa. The expected band size for Hes1 is at 30 kDa.
Product group Antibodies
References
Boster Bio
Anti-Hes1 Rabbit Monoclonal AntibodyM01459
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetHES1
  • SizePrice
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus.
Product group Antibodies
Atlas Antibodies
Anti-HES1 AntibodyHPA066929
ApplicationsWestern Blot, ImmunoCytoChemistry
ReactivityHuman
TargetHES1
  • SizePrice
Product group Antibodies
Biomatik
Hairy And Enhancer Of Split 1 (HES1) Polyclonal AntibodyCAU21580
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityMouse
TargetHES1
  • SizePrice
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with HES1 Polyclonal Antibody, Unconjugated (bs-2972R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Product group Antibodies
References
Bioss
HES1 Polyclonal AntibodyBS-2972R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityBovine, Equine, Human, Mouse, Porcine, Rabbit, Rat
TargetHES1
  • SizePrice
IHC-P analysis of breast tissue using GTX84375 HES1 antibody [4H1]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.
Product group Antibodies
GeneTex
HES1 antibody [4H1]GTX84375
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetHES1
  • SizePrice
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HES1 antibody [N1], N-term (GTX108356) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Product group Antibodies
GeneTex
HES1 antibody [N1], N-termGTX108356
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetHES1
  • SizePrice