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Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using HGS(GTX101738) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using HGS(GTX101738) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using HGS(GTX101738) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

HGS antibody

GTX101738
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetHGS
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Overview

  • Supplier
    GeneTex
  • Product Name
    HGS antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.15 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID9146
  • Target name
    HGS
  • Target description
    hepatocyte growth factor-regulated tyrosine kinase substrate
  • Target synonyms
    HRS, hepatocyte growth factor-regulated tyrosine kinase substrate, human growth factor-regulated tyrosine kinase substrate, protein pp110
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO14964
  • Protein Name
    Hepatocyte growth factor-regulated tyrosine kinase substrate
  • Scientific Description
    The protein encoded by this gene regulates endosomal sorting and plays a critical role in the recycling and degradation of membrane receptors. The encoded protein sorts monoubiquitinated membrane proteins into the multivesicular body, targeting these proteins for lysosome-dependent degradation. [provided by RefSeq]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Scotti E, Calamai M, Goulbourne CN, et al. IDOL stimulates clathrin-independent endocytosis and multivesicular body-mediated lysosomal degradation of the low-density lipoprotein receptor. Mol Cell Biol. 2013,33(8):1503-14. doi: 10.1128/MCB.01716-12
    Read this paper

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Figure 1. Western blot analysis of HGS using anti-HGS antibody (A01174-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human SK-OV-3 whole cell lysates, Lane 5: human PC-3 whole cell lysates, Lane 6: human A549 whole cell lysates, Lane 7: human HepG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HGS antigen affinity purified polyclonal antibody (Catalog # A01174-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HGS at approximately 110-115KD. The expected band size for HGS is at 110-115KD.
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