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HeLa cells probed with HIF-1 Alpha Polyclonal Antibody, unconjugated (bs-0737R) at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange)
HeLa cells probed with HIF-1 Alpha Polyclonal Antibody, unconjugated (bs-0737R) at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange)
HeLa cells probed with HIF-1 Alpha Polyclonal Antibody, unconjugated (bs-0737R) at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange)

HIF-1 Alpha Polyclonal Antibody

BS-0737R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetHIF1A
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Overview

  • Supplier
    Bioss
  • Product Name
    HIF-1 Alpha Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200), IP(1-2ug)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID3091
  • Target name
    HIF1A
  • Target description
    hypoxia inducible factor 1 subunit alpha
  • Target synonyms
    HIF-1-alpha, HIF-1A, HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78, hypoxia-inducible factor 1-alpha, ARNT interacting protein, PAS domain-containing protein 8, basic-helix-loop-helix-PAS protein MOP1, class E basic helix-loop-helix protein 78, hypoxia inducible factor 1 alpha subunit, hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor), hypoxia-inducible factor1alpha, member of PAS protein 1, member of PAS superfamily 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ16665
  • Protein Name
    Hypoxia-inducible factor 1-alpha
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Sun Y, Li M, Liu G, et al. The function of Piezo1 in colon cancer metastasis and its potential regulatory mechanism. J Cancer Res Clin Oncol. 2020,146(5):1139-1152. doi: 10.1007/s00432-020-03179-w
    Read this paper
  • Ouyang H, Tan Y, Li Q, et al. RETRACTED: MicroRNA-208-5p regulates myocardial injury of sepsis mice via targeting SOCS2-mediated NF-κB/HIF-1α pathway. Int Immunopharmacol. 2020,81:106204. doi: 10.1016/j.intimp.2020.106204
    Read this paper
  • Duda P, Janczara J, McCubrey JA, et al. The Reverse Warburg Effect is Associated with Fbp2-Dependent Hif1α Regulation in Cancer Cells Stimulated by Fibroblasts. Cells. 2020,9(1). doi: 10.3390/cells9010205
    Read this paper
  • Wu TH, Yeh KY, Wang CH, et al. The Combination of Astragalus membranaceus and Angelica sinensis Inhibits Lung Cancer and Cachexia through Its Immunomodulatory Function. J Oncol. 2019,2019:9206951. doi: 10.1155/2019/9206951
    Read this paper
  • Shen Y, Li S, Wang X, et al. Tumor vasculature remolding by thalidomide increases delivery and efficacy of cisplatin. J Exp Clin Cancer Res. 2019,38(1):427. doi: 10.1186/s13046-019-1366-x
    Read this paper
  • Li ZH, Wu C, Ke H, et al. You-Gui-Yin improved the reproductive dysfunction of male rats with chronic kidney disease via regulating the HIF1α-STAT5 pathway. J Ethnopharmacol. 2020,246:112240. doi: 10.1016/j.jep.2019.112240
    Read this paper
  • Wang D, Zhu H, Cheng W, et al. Effects of hypoxia and ASIC3 on nucleus pulposus cells: From cell behavior to molecular mechanism. Biomed Pharmacother. 2019,117:109061. doi: 10.1016/j.biopha.2019.109061
    Read this paper
  • Yang X, Wang F, Zhu H, et al. Synthesis and Bioevaluation of Novel [(18)F]FDG-Conjugated 2-Nitroimidazole Derivatives for Tumor Hypoxia Imaging. Mol Pharm. 2019,16(5):2118-2128. doi: 10.1021/acs.molpharmaceut.9b00075
    Read this paper
  • Yang Z, Zhang C, Feng Y, et al. Tenascin-C is involved in promotion of cancer stemness via the Akt/HIF1ɑ axis in esophageal squamous cell carcinoma. Exp Mol Pathol. 2019,109:104239. doi: 10.1016/j.yexmp.2019.03.007
    Read this paper
  • Chai D, Li K, Du H, et al. β2-microglobulin has a different regulatory molecular mechanism between ER(+) and ER(-) breast cancer with HER2(). BMC Cancer. 2019,19(1):223. doi: 10.1186/s12885-019-5410-1
    Read this paper

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Figure 1. Western blot analysis of HIF1A using anti-HIF1A antibody (A00013-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human U2OS whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: human HepG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIF1A antigen affinity purified polyclonal antibody (Catalog # A00013-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIF1A at approximately 123KD. The expected band size for HIF1A is at 93KD.
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