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ProductsBack/Histone H1 (Pan Nuclear Marker) Antibody

Histone H1 (Pan Nuclear Marker) Antibody

ORB2319016
Biorbyt
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetH1-0
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Overview

  • Supplier
    Biorbyt
  • Product Name
    Histone H1 (Pan Nuclear Marker) Antibody
  • Delivery Days Customer
    10
  • Application Supplier Note
    Positive Control: HeLa, A-431, LNCap or Jurkat cells. Breast carcinoma. Human heart tissue lysate.. Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); Western Blot (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes);Optimal dilution for a specific application should be determined. FC, IF, IHC, WB
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    SPM256
  • Concentration
    200 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID3005
  • Target name
    H1-0
  • Target description
    H1.0 linker histone
  • Target synonyms
    H1.0, H10, H1F0, H1FV, histone H1.0, H1 histone family member 0, H1.0, H1(0), H1-0, histone H1', histone H1(0)
  • Host
    Mouse
  • Isotype
    IgG2a
  • Scientific Description
    Histone H1 (Pan Nuclear Marker) Antibody
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Histone H1.0/H1F0 using anti-Histone H1.0/H1F0 antibody (A08821-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U20S whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Histone H1.0/H1F0 antigen affinity purified polyclonal antibody (Catalog # A08821-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Histone H1.0/H1F0 at approximately 24 kDa. The expected band size for Histone H1.0/H1F0 is at 21 kDa.
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