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HLA-DRB1 Antibody

LS-C409239
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
TargetHLA-DRB1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    HLA-DRB1 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 30kDa, while the observed MW by Western blot was 30kDa.
  • Applications
    ImmunoFluorescence, Western Blot
  • Applications Supplier
    IF (1:50 - 1:100), WB (1:500 - 1:2000) The predicted MW is 30kDa, while the observed MW by Western blot was 30kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.192 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID3123
  • Target name
    HLA-DRB1
  • Target description
    major histocompatibility complex, class II, DR beta 1
  • Target synonyms
    DRB1, HLA-DR1B, HLA-DRB, SS1, major histocompatibility complex, class II, DR beta 1 precursor, HLA class II histocompatibility antigen, DR-1 beta chain, MHC class II HLA-DR beta 1 chain, human leucocyte antigen DRB1, lymphocyte antigen DRB1
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HLA Class II DRB1 using anti-HLA Class II DRB1 antibody (A00568). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Raji whole cell lysate, Lane 2: human Raji whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HLA Class II DRB1 antigen affinity purified polyclonal antibody (Catalog # A00568) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HLA Class II DRB1 at approximately 30KD. The expected band size for HLA Class II DRB1 is at 30KD.
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