Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [HL2059] (GTX637963) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![Indirect ELISA analysis was performed by coating the plate with recombinant HEK293 expressed, human HMGB1 protein (1000-15.63 ng/mL). Coated protein was probed with HMGB1 antibody [HL2059] (GTX637963) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637963/GTX637963_T-44886_20220113_ELISA_Indirect_23011618_221.webp "Indirect ELISA analysis was performed by coating the plate with recombinant HEK293 expressed, human HMGB1 protein (1000-15.63 ng/mL). Coated protein was probed with HMGB1 antibody [HL2059] (GTX637963) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.")
![Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [HL2059] (GTX637963) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637963/GTX637963_44935_20230203_WB_23020621_399.webp "Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [HL2059] (GTX637963) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![HMGB1 antibody [HL2059] detects HMGB1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. HMGB1 stained by HMGB1 antibody [HL2059] (GTX637963) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX637963/GTX637963_T-44886_20221230_IHC-P_multiple_M_23020621_380.webp "HMGB1 antibody [HL2059] detects HMGB1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. HMGB1 stained by HMGB1 antibody [HL2059] (GTX637963) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![HMGB1 antibody [HL2059] detects HMGB1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HMGB1 stained by HMGB1 antibody [HL2059] (GTX637963) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637963/GTX637963_T-44886_20230203_ICC_IF_23021401_804.webp "HMGB1 antibody [HL2059] detects HMGB1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HMGB1 stained by HMGB1 antibody [HL2059] (GTX637963) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.")
![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [HL2059] (GTX637963) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637963/GTX637963_44935_20230210_WB_shRNA_watermark_23021401_300.webp "Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [HL2059] (GTX637963) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![HMGB1 antibody [HL2059] detects HMGB1 protein at nucleus by immunofluorescent analysis. Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HMGB1 stained by HMGB1 antibody [HL2059] (GTX637963) diluted at 1:250. Red: Tau, an axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637963/GTX637963_T-44886_20230324_ICC_IF_R_23041023_472.webp "HMGB1 antibody [HL2059] detects HMGB1 protein at nucleus by immunofluorescent analysis. Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HMGB1 stained by HMGB1 antibody [HL2059] (GTX637963) diluted at 1:250. Red: Tau, an axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.")
![Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [HL2059] (GTX637963) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637963/GTX637963_44935_20230616_WB_D_23062019_603.webp "Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [HL2059] (GTX637963) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![HMGB1 antibody [HL2059] detects HMGB1 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded cat kidney. HMGB1 stained by HMGB1 antibody [HL2059] (GTX637963) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX637963/GTX637963_44935_20230721_IHC-P_Cat_23073119_408.webp "HMGB1 antibody [HL2059] detects HMGB1 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded cat kidney. HMGB1 stained by HMGB1 antibody [HL2059] (GTX637963) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Non-transfected (–) and transfected (+) 293T whole cell extracts (5 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [HL2059] (GTX637963) diluted at 1:300000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637963/GTX637963_44935_20230901_WB_multiple_B_23090619_386.webp "Non-transfected (–) and transfected (+) 293T whole cell extracts (5 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [HL2059] (GTX637963) diluted at 1:300000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [HL2059] (GTX637963) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
HMGB1 antibody [HL2059]
GTX637963
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityCanine, Feline, Human, Mouse, Rat
TargetHMGB1
Overview
- SupplierGeneTex
- Product NameHMGB1 antibody [HL2059]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL2059
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID3146
- Target nameHMGB1
- Target descriptionhigh mobility group box 1
- Target synonymsHMG-1, HMG1, HMG3, SBP-1, high mobility group protein B1, Amphoterin, Sulfoglucuronyl carbohydrate binding protein, high-mobility group (nonhistone chromosomal) protein 1
- HostRabbit
- IsotypeIgG
- Protein IDP09429
- Protein NameHigh mobility group protein B1
- Scientific DescriptionThis gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2015]
- ReactivityCanine, Feline, Human, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
Datasheet
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![Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [HL2060] (GTX637964) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637964/GTX637964_T-44886_20230116_WB_23011618_157.webp)
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