Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HNF1 alpha antibody [HL3229] (GTX640870) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
![Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HNF1 alpha antibody [HL3229] (GTX640870) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX640870/GTX640870_T-45509_20240906_WB_M_liver_24091102_677.webp "Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HNF1 alpha antibody [HL3229] (GTX640870) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![HNF1 alpha antibody [HL3229] detects HNF1 alpha protein by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HNF1 alpha stained by HNF1 alpha antibody [HL3229] (GTX640870) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.](https://www.genetex.com/upload/website/prouct_img/normal/GTX640870/GTX640870_T-45509_20240906_ICC_IF_24091102_256.webp "HNF1 alpha antibody [HL3229] detects HNF1 alpha protein by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HNF1 alpha stained by HNF1 alpha antibody [HL3229] (GTX640870) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.")
![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HNF1 alpha antibody [HL3229] (GTX640870) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX640870/GTX640870_T-45509_20240906_WB_multiple_B_24091102_203.webp "Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HNF1 alpha antibody [HL3229] (GTX640870) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![HNF1 alpha antibody [HL3229] detects HNF1 alpha protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. HNF1 alpha stained by HNF1 alpha antibody [HL3229] (GTX640870) diluted at 1:100. Antigen Retrieval: Tris-EDTA-Citrate acid buffer, pH 7.8, 15 min Corresponding RNA expression data for the same tissues are based on NCBI.](https://www.genetex.com/upload/website/prouct_img/normal/GTX640870/GTX640870_T-45509_20241018_IHC-P_Mutiple_M_24111321_642.webp "HNF1 alpha antibody [HL3229] detects HNF1 alpha protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. HNF1 alpha stained by HNF1 alpha antibody [HL3229] (GTX640870) diluted at 1:100. Antigen Retrieval: Tris-EDTA-Citrate acid buffer, pH 7.8, 15 min Corresponding RNA expression data for the same tissues are based on NCBI.")
![HNF1 alpha antibody [HL3229] detects HNF1 alpha protein by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. HNF1 alpha stained by HNF1 alpha antibody [HL3229] (GTX640870) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX640870/GTX640870_T-45509_20241018_IHC-P_24111321_287.webp "HNF1 alpha antibody [HL3229] detects HNF1 alpha protein by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. HNF1 alpha stained by HNF1 alpha antibody [HL3229] (GTX640870) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HNF1 alpha antibody [HL3229] (GTX640870) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
HNF1 alpha antibody [HL3229]
GTX640870
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetHNF1A
Overview
- SupplierGeneTex
- Product NameHNF1 alpha antibody [HL3229]
- Delivery Days Customer7
- Application Supplier NoteWB: 1:500-1:10000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL3229
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID6927
- Target nameHNF1A
- Target descriptionHNF1 homeobox A
- Target synonymsHNF-1-alpha, HNF-1A, HNF1, HNF1alpha, HNF4A, IDDM20, LFB1, MODY3, TCF-1, TCF1, hepatocyte nuclear factor 1-alpha, albumin proximal factor, hepatic nuclear factor 1, interferon production regulator factor, liver-specific transcription factor LF-B1, transcription factor 1, hepatic
- HostRabbit
- IsotypeIgG
- Protein IDP20823
- Protein NameHepatocyte nuclear factor 1-alpha
- Scientific DescriptionThe protein encoded by this gene is a transcription factor required for the expression of several liver-specific genes. The encoded protein functions as a homodimer and binds to the inverted palindrome 5-GTTAATNATTAAC-3. Defects in this gene are a cause of maturity onset diabetes of the young type 3 (MODY3) and also can result in the appearance of hepatic adenomas. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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![HNF1 alpha antibody [N1N3] detects HNF1 alpha protein at nucleus by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HNF1 alpha stained by HNF1 alpha antibody [N1N3] (GTX113850) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Scale bar= 10μm.](https://www.genetex.com/upload/website/prouct_img/normal/GTX113850/GTX113850_44665_20220624_ICC_IF_22062919_549.webp)
Product group Antibodies
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ReactivityHuman, Mouse
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