
Sample (30 μg of whole cell lysate) A: HepG2 7.5% SDS PAGE GTX628240 diluted at 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
HNF1 alpha antibody [GT4110]
GTX628240
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetHNF1A
Overview
- SupplierGeneTex
- Product NameHNF1 alpha antibody [GT4110]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT4110
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID6927
- Target nameHNF1A
- Target descriptionHNF1 homeobox A
- Target synonymsHNF-1-alpha, HNF-1A, HNF1, HNF1alpha, HNF4A, IDDM20, LFB1, MODY3, TCF-1, TCF1, hepatocyte nuclear factor 1-alpha, albumin proximal factor, hepatic nuclear factor 1, interferon production regulator factor, liver-specific transcription factor LF-B1, transcription factor 1, hepatic
- HostMouse
- IsotypeIgG2a
- Protein IDP20823
- Protein NameHepatocyte nuclear factor 1-alpha
- Scientific DescriptionThe protein encoded by this gene is a transcription factor required for the expression of several liver-specific genes. The encoded protein functions as a homodimer and binds to the inverted palindrome 5-GTTAATNATTAAC-3. Defects in this gene are a cause of maturity onset diabetes of the young type 3 (MODY3) and also can result in the appearance of hepatic adenomas. [provided by RefSeq]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
![Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HNF1 alpha antibody [GT4110] (GTX628240) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HNF1 alpha antibody [GT4110] (GTX628240) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX628240/GTX628240_41057_20160204_WB_M_liver_w_23061202_385.webp)
![HNF1 alpha antibody [GT4110] detects HNF1 alpha protein at nucleus in human SW480 by immunohistochemical analysis. Sample: Paraffin-embedded human SW480. HNF1 alpha antibody [GT4110] (GTX628240) diluted at 1:250.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min HNF1 alpha antibody [GT4110] detects HNF1 alpha protein at nucleus in human SW480 by immunohistochemical analysis. Sample: Paraffin-embedded human SW480. HNF1 alpha antibody [GT4110] (GTX628240) diluted at 1:250.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX628240/GTX628240_41057_20160616_IHC-P_w_23061202_559.webp)






![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HNF1 alpha antibody [HL3229] (GTX640870) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](https://www.genetex.com/upload/website/prouct_img/normal/GTX640870/GTX640870_T-45509_20240906_WB_TPM_watermark_24091102_352.webp)
![HNF1 alpha antibody [N1N3] detects HNF1 alpha protein at nucleus by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HNF1 alpha stained by HNF1 alpha antibody [N1N3] (GTX113850) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Scale bar= 10μm.](https://www.genetex.com/upload/website/prouct_img/normal/GTX113850/GTX113850_44665_20220624_ICC_IF_22062919_549.webp)