![WB analysis of HEK293 (1) and IGF2 (AA: 25-180)-hIgGFc transfected HEK293 (2) cell lysate using GTX60630 IGF2 antibody [8H1]. WB analysis of HEK293 (1) and IGF2 (AA: 25-180)-hIgGFc transfected HEK293 (2) cell lysate using GTX60630 IGF2 antibody [8H1].](https://www.genetex.com/upload/website/prouct_img/normal/GTX60630/GTX60630_20170912_WB_w_23061123_506.webp)
WB analysis of HEK293 (1) and IGF2 (AA: 25-180)-hIgGFc transfected HEK293 (2) cell lysate using GTX60630 IGF2 antibody [8H1].
IGF2 antibody [8H1]
GTX60630
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetIGF2
Overview
- SupplierGeneTex
- Product NameIGF2 antibody [8H1]
- Delivery Days Customer9
- Application Supplier NoteWB: 1/500 - 1/2000. ICC/IF: 1/200 - 1/1000. IHC-P: 1/200 - 1/1000. FACS: 1/200 - 1/400. ELISA: 1/10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID8H1
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID3481
- Target nameIGF2
- Target descriptioninsulin like growth factor 2
- Target synonymsC11orf43, GRDF, IGF-II, PP9974, SRS3, insulin-like growth factor 2, T3M-11-derived growth factor, insulin-like growth factor 2 (somatomedin A), insulin-like growth factor type 2, preptin
- HostMouse
- IsotypeIgG1
- Protein IDP01344
- Protein NameInsulin-like growth factor 2
- Scientific DescriptionThis gene encodes a member of the insulin family of polypeptide growth factors, which are involved in development and growth. It is an imprinted gene, expressed only from the paternal allele, and epigenetic changes at this locus are associated with Wilms tumour, Beckwith-Wiedemann syndrome, rhabdomyosarcoma, and Silver-Russell syndrome. A read-through INS-IGF2 gene exists, whose 5 region overlaps the INS gene and the 3 region overlaps this gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2010]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Hsu CF, Huang HS, Chen PC, et al. IGF-axis confers transformation and regeneration of fallopian tube fimbria epithelium upon ovulation. EBioMedicine. 2019,41:597-609. doi: 10.1016/j.ebiom.2019.01.061Read this paper
![FACS analysis of HepG2 cells using GTX60630 IGF2 antibody [8H1]. Green : IGF2 Red : negative control FACS analysis of HepG2 cells using GTX60630 IGF2 antibody [8H1]. Green : IGF2 Red : negative control](https://www.genetex.com/upload/website/prouct_img/normal/GTX60630/GTX60630_20170912_FACS_w_23061123_458.webp)
![ELISA analysis of antigen using GTX60630 IGF2 antibody [8H1].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng ELISA analysis of antigen using GTX60630 IGF2 antibody [8H1].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng](https://www.genetex.com/upload/website/prouct_img/normal/GTX60630/GTX60630_20170912_ELISA_w_23061123_493.webp)
![IHC-P analysis of ovarian cancer tissue using GTX60630 IGF2 antibody [8H1]. IHC-P analysis of ovarian cancer tissue using GTX60630 IGF2 antibody [8H1].](https://www.genetex.com/upload/website/prouct_img/normal/GTX60630/GTX60630_20170912_IHC-P_1_w_23061123_839.webp)
![ICC/IF analysis of HeLa cells using GTX60630 IGF2 antibody [8H1]. Green : IGF2 Blue: DRAQ5 fluorescent DNA dye Red: Actin filaments ICC/IF analysis of HeLa cells using GTX60630 IGF2 antibody [8H1]. Green : IGF2 Blue: DRAQ5 fluorescent DNA dye Red: Actin filaments](https://www.genetex.com/upload/website/prouct_img/normal/GTX60630/GTX60630_20170912_ICCIF_w_23061123_250.webp)
![IHC-P analysis of bladder cancer tissue using GTX60630 IGF2 antibody [8H1]. IHC-P analysis of bladder cancer tissue using GTX60630 IGF2 antibody [8H1].](https://www.genetex.com/upload/website/prouct_img/normal/GTX60630/GTX60630_20170912_IHC-P_w_23061123_398.webp)

![HepG2 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IGF2 antibody [N1C3] (GTX100453) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX100453/GTX100453_44517_20230203_WB_Fraction_23020621_996.webp)
![Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with IGF2 antibody [HL1979] (GTX637872) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637872/GTX637872_T-44865_20230127_WB_M_placenta_23013122_169.webp)
![Mouse plasma (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with IGF2 antibody [HL1982] (GTX637875) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637875/GTX637875_T-44865_20221230_WB_M_plasma_23010400_892.webp)
![HepG2 whole cell extract and conditioned medium (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IGF2 antibody [HL2132] (GTX638104) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638104/GTX638104_44998_20230331_WB_Fraction_23041023_266.webp)

