HepG2 whole cell extract and conditioned medium (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IGF2 antibody [HL2132] (GTX638104) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![Recombinant IGF2 protein were separated by 15% SDS-PAGE, and the membrane was blotted with IGF2 antibody [HL2132] (GTX638104) diluted at 1:2000. The HRP-conjated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638104/GTX638104_44998_20230414_WB_Ag_23041723_301.webp "Recombinant IGF2 protein were separated by 15% SDS-PAGE, and the membrane was blotted with IGF2 antibody [HL2132] (GTX638104) diluted at 1:2000. The HRP-conjated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Indirect ELISA analysis was performed by coating the plate with recombinant E.coli expressed, mature recombinant human IGF2 protein (533.33-8.33 nM). Coated protein was probed with IGF2 antibody [HL2132] (GTX638104) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638104/GTX638104_44998_20230512_ELISA_Indirect_23051702_722.webp "Indirect ELISA analysis was performed by coating the plate with recombinant E.coli expressed, mature recombinant human IGF2 protein (533.33-8.33 nM). Coated protein was probed with IGF2 antibody [HL2132] (GTX638104) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.")
![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with IGF2 antibody [HL2132] (GTX638104) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638104/GTX638104_44998_20230526_WB_multiple_B_23053001_192.webp "Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with IGF2 antibody [HL2132] (GTX638104) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Sandwich ELISA detection of recombinant E.coli expressed, mature recombinant human IGF2 protein using antibodies as below. Capture: IGF2 antibody [HL2937] (GTX640321) (5 μg/mL) Detection: HRP-conjugated IGF2 antibody [HL2132] (GTX638104) (1 μg/mL) Please notice that GTX638104 needs to be conjugated to HRP to function as the detection antibody when paired with GTX640321. Please contact us for custom HRP-conjugated antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638104/GTX638104_44998_20240510_ELISA_PAIR_24051400_801.webp "Sandwich ELISA detection of recombinant E.coli expressed, mature recombinant human IGF2 protein using antibodies as below. Capture: IGF2 antibody [HL2937] (GTX640321) (5 μg/mL) Detection: HRP-conjugated IGF2 antibody [HL2132] (GTX638104) (1 μg/mL) Please notice that GTX638104 needs to be conjugated to HRP to function as the detection antibody when paired with GTX640321. Please contact us for custom HRP-conjugated antibody.")
HepG2 whole cell extract and conditioned medium (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IGF2 antibody [HL2132] (GTX638104) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
IGF2 antibody [HL2132]
GTX638104
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetIGF2
Overview
- SupplierGeneTex
- Product NameIGF2 antibody [HL2132]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ELISA
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL2132
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID3481
- Target nameIGF2
- Target descriptioninsulin like growth factor 2
- Target synonymsC11orf43, GRDF, IGF-II, PP9974, SRS3, insulin-like growth factor 2, T3M-11-derived growth factor, insulin-like growth factor 2 (somatomedin A), insulin-like growth factor type 2, preptin
- HostRabbit
- IsotypeIgG
- Protein IDP01344
- Protein NameInsulin-like growth factor 2
- Scientific DescriptionThis gene encodes a member of the insulin family of polypeptide growth factors, which are involved in development and growth. It is an imprinted gene, expressed only from the paternal allele, and epigenetic changes at this locus are associated with Wilms tumour, Beckwith-Wiedemann syndrome, rhabdomyosarcoma, and Silver-Russell syndrome. A read-through INS-IGF2 gene exists, whose 5 region overlaps the INS gene and the 3 region overlaps this gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2010]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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