More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/IHC-plus(tm) AFAP1 / AFAP Antibody

IHC-plus(tm) AFAP1 / AFAP Antibody

LS-B9578
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetAFAP1
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) AFAP1 / AFAP Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    AFAP1 antibody can be used for detection of AFAP1 by Western blot at 1 - 2 ug/mL. Antibody validated: Western Blot in human samples. All other applications and species not yet tested. Western Blot:
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA, IHC, IHC-P (5 µg/ml), WB (1 - 2 µg/ml) AFAP1 antibody can be used for detection of AFAP1 by Western blot at 1 - 2 ug/mL. Antibody validated: Western Blot in human samples. All other applications and species not yet tested. Western Blot:
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID60312
  • Target name
    AFAP1
  • Target description
    actin filament associated protein 1
  • Target synonyms
    AFAP, AFAP-110, AFAP110, actin filament-associated protein 1, 110 kDa actin filament-associated protein, actin filament-associated protein, 110 kDa
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
RayBiotech
Anti-AFAP1 Antibody144-03478
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetAFAP1
  • SizePrice
Product group Antibodies
Bioss
AFAP Polyclonal AntibodyBS-9790R
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityBovine, Canine, Equine, Human, Mouse, Rat, Sheep
TargetAFAP1
  • SizePrice
Product group Antibodies
Assay Genie
AFAP1 AntibodyCAB3478
ReactivityHuman
TargetAFAP1
  • SizePrice
Anti-AFAP1 Antibody
Product group Antibodies
Antibodies.com
Anti-AFAP1 AntibodyA37858
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
  • SizePrice
WB analysis of HeLa cell lysate using GTX31462 AFAP antibody. Working concentration : (A) 1 and (B) 2 μg/ml
Product group Antibodies
GeneTex
AFAP antibodyGTX31462
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse
TargetAFAP1
  • SizePrice
Figure 1. Western blot analysis of AFAP/AFAP1 using anti-AFAP/AFAP1 antibody (A05258-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human U-87MG whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat lung tissue lysates, Lane 7: rat stomach tissue lysates, Lane 8: mouse brain tissue lysates, Lane 9: mouse lung tissue lysates, Lane 10: mouse stomach tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AFAP/AFAP1 antigen affinity purified polyclonal antibody (Catalog # A05258-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AFAP/AFAP1 at approximately 120KD. The expected band size for AFAP/AFAP1 is at 120KD.
Product group Antibodies
Boster Bio
Anti-AFAP/AFAP1 Antibody Picoband(r)A05258-3-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetAFAP1
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
AFAP1 / AFAP AntibodyLS-C332595
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetAFAP1
  • SizePrice
The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using CSB-PA098902(AFAP1 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: x200)
Product group Antibodies
Cusabio
AFAP1 AntibodyCSB-PA098902
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetAFAP1
  • SizePrice
Immunohistochemistry analysis in human colon and liver tissues using HPA015642 antibody. Corresponding AFAP1 RNA-seq data are presented for the same tissues.
Product group Antibodies
Atlas Antibodies
Anti-AFAP1 AntibodyHPA015642
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetAFAP1
  • SizePrice