IHC-plus(tm) AKT1 Antibody (phospho-Thr308)

LifeSpan BioSciences

IHC-plus(tm) AKT1 Antibody (phospho-Thr308)

23

Immunohistochemistry: LS-B1190 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1190 was determined to be 5 ug/ml.. ELISA (1:15000), Flo, IHC, IHC-P (5 µg/ml), WB (1:1000) Immunohistochemistry: LS-B1190 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1190 was determined to be 5 ug/ml.

Flow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

0.97 mg/ml

Unconjugated

AKT1

AKT serine/threonine kinase 1

AKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA, RAC-alpha serine/threonine-protein kinase, AKT1m, PKB alpha, RAC-PK-alpha, protein kinase B alpha, proto-oncogene c-Akt, rac protein kinase alpha, serine-threonine protein kinase, v-akt murine thymoma viral oncogene homolog 1, v-akt murine thymoma viral oncogene-like protein 1

Rabbit

IgG

Human, Mouse, Rat

2°C to 8°C or -20°C

41116161