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ProductsBack/IHC-plus(tm) CALR / Calreticulin Antibody (aa291-304)

IHC-plus(tm) CALR / Calreticulin Antibody (aa291-304)

LS-B11268
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Monkey, Rat
TargetCALR
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) CALR / Calreticulin Antibody (aa291-304)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 60kD band observed in Human and Rat Lung lysates (calculated MW of 48.1kD according to NP_004334.1). These bands correspond to earlier findings in literature with different antibodies (Conway et al, J Biol Chem. 1995 Jul 14.270(28):17011-6. PMID: 7622522). Recommended concentration: 0.01-0.03 ug/ml.. IHC, IHC-P (5 µg/ml), Peptide-ELISA (1:128000), WB (0.01 - 0.03 µg/ml) Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 60kD band observed in Human and Rat Lung lysates (calculated MW of 48.1kD according to NP_004334.1). These bands correspond to earlier findings in literature with different antibodies (Conway et al, J Biol Chem. 1995 Jul 14.270(28):17011-6. PMID: 7622522). Recommended concentration: 0.01-0.03 ug/ml.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID811
  • Target name
    CALR
  • Target description
    calreticulin
  • Target synonyms
    CALR1, CRT, HEL-S-99n, RO, SSA, cC1qR, calreticulin, CRP55, ERp60, HACBP, Sicca syndrome antigen A (autoantigen Ro; calreticulin), calregulin, endoplasmic reticulum resident protein 60, epididymis secretory sperm binding protein Li 99n, grp60
  • Host
    Goat
  • Reactivity
    Human, Monkey, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CALR using anti-CALR antibody (A00894-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human U-937 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human THP-1 whole cell lysates, Lane 6: human T-47D whole cell lysates, Lane 7: human PC-3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CALR antigen affinity purified polyclonal antibody (Catalog # A00894-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CALR at approximately 60KD. The expected band size for CALR is at 48KD.
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