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ProductsBack/IHC-plus(tm) CCR3 Antibody (N-Terminus)

IHC-plus(tm) CCR3 Antibody (N-Terminus)

LS-B5218
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCCR3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) CCR3 Antibody (N-Terminus)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Suitable for use in Western Blot. Western Blot: 1-2 ug/ml. Positive control: Human spleen cell lysate.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-P (5 µg/ml), WB (1 - 2 µg/ml) Suitable for use in Western Blot. Western Blot: 1-2 ug/ml. Positive control: Human spleen cell lysate.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1232
  • Target name
    CCR3
  • Target description
    C-C motif chemokine receptor 3
  • Target synonyms
    C C CKR3, CC-CKR-3, CD193, CKR 3, CKR3, CMKBR3, C-C chemokine receptor type 3, C-C CKR-3, CC chemokine receptor 3, CCR-3, b-chemokine receptor, chemokine (C-C motif) receptor 3, eosinophil CC chemokine receptor 3, eosinophil eotaxin receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of CCR3 using anti-CCR3 antibody (A01748-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human U-87MG whole cell lysates, Lane 5: human CCRF-CEM whole cell lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCR3 antigen affinity purified polyclonal antibody (Catalog # A01748-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCR3 at approximately 55KD. The expected band size for CCR3 is at 41KD.
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