IHC-plus(tm) CLDN5 / Claudin 5 Antibody

LifeSpan BioSciences

IHC-plus(tm) CLDN5 / Claudin 5 Antibody

23

Immunohistochemistry: LS-B2331 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2331 was determined to be 5-10 ug/ml. Positive control: Rat lung, kidney, and small intestine homogenates and mouse lung homogenates.. ELISA (0.1 - 1 µg/ml), IHC, IHC-P (5 - 10 µg/ml), WB (1 - 3 µg/ml) Immunohistochemistry: LS-B2331 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2331 was determined to be 5-10 ug/ml. Positive control: Rat lung, kidney, and small intestine homogenates and mouse lung homogenates.

Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Monoclonal

0.5 mg/ml

Unconjugated

CLDN5

claudin 5

AWAL, BEC1, CPETRL1, TMDVCF, TMVCF, claudin-5, transmembrane protein deleted in VCFS, transmembrane protein deleted in velocardiofacial syndrome

Mouse

IgG1

Mouse, Rat

-20°C,2°C to 8°C

41116161