IHC-plus(tm) ICAM-1 / CD54 Antibody

LifeSpan BioSciences

IHC-plus(tm) ICAM-1 / CD54 Antibody

23

Immunohistochemistry: LS-B2647 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2647 was determined to be 10 ug/ml. Flow Cytometry: 1:50-1:100. Use 10 ul to label 10^6 cells in 100ul. Positive control: Human Tonsil.. Flo (1:50 - 1:100), IHC, IHC-Fr (1:50 - 1:100), IHC-P (10 µg/ml), IP Immunohistochemistry: LS-B2647 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2647 was determined to be 10 ug/ml. Flow Cytometry: 1:50-1:100. Use 10 ul to label 10^6 cells in 100ul. Positive control: Human Tonsil.

Flow Cytometry, ImmunoPrecipitation, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin

Research Use Only

Monoclonal

1 mg/ml

Unconjugated

ICAM1

intercellular adhesion molecule 1

BB2, CD54, P3.58, intercellular adhesion molecule 1, ICAM-1, cell surface glycoprotein P3.58, epididymis secretory sperm binding protein, intercellular adhesion molecule 1 (CD54), human rhinovirus receptor, major group rhinovirus receptor

Mouse

IgG1

Human

-20°C,2°C to 8°C

41116161