IHC-plus(tm) MAG Antibody (C-Terminus)

LifeSpan BioSciences

IHC-plus(tm) MAG Antibody (C-Terminus)

23

Immunohistochemistry: LS-B2213 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2213 was determined to be 5 ug/ml. Immunoprecipitation: 1-3 ug/IP reaction. Immunofluorescence: 10-30 ug/ul. Positive control: Rat brain homogenates.. IF, IHC, IHC-P (5 µg/ml), IP, WB (1 - 3 µg/ml) Immunohistochemistry: LS-B2213 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2213 was determined to be 5 ug/ml. Immunoprecipitation: 1-3 ug/IP reaction. Immunofluorescence: 10-30 ug/ul. Positive control: Rat brain homogenates.

ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

0.25 mg/ml

Unconjugated

MAG

myelin associated glycoprotein

GMA, S-MAG, SIGLEC-4A, SIGLEC4, SIGLEC4A, SPG75, myelin-associated glycoprotein, sialic acid binding Ig-like lectin 4A, sialic acid-binding immunoglobulin-like lectin 4A

Rabbit

Human, Rat

-20°C,2°C to 8°C

41116161