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ProductsBack/IHC-plus(tm) MYH14 Antibody (aa1051-1100)

IHC-plus(tm) MYH14 Antibody (aa1051-1100)

LS-B9846
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetMYH14
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) MYH14 Antibody (aa1051-1100)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Western Blot: Predicted MW 228 kDa
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-P (1:50), Peptide-ELISA (1:5000), WB (1:500 - 1:1000) Western Blot: Predicted MW 228 kDa
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID79784
  • Target name
    MYH14
  • Target description
    myosin heavy chain 14
  • Target synonyms
    DFNA4, DFNA4A, FP17425, MHC16, MYH17, NMHC II-C, NMHC-II-C, PNMHH, myosin, myosin-14, MYH14 variant protein, myosin heavy chain, non-muscle IIc, myosin, heavy chain 14, non-muscle, myosin, heavy polypeptide 14, non-muscle myosin heavy chain IIc, nonmuscle myosin heavy chain II-C
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MYH14 using anti-MYH14 antibody (A04528-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: rat lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYH14 antigen affinity purified polyclonal antibody (Catalog # A04528-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MYH14 at approximately 250 kDa. The expected band size for MYH14 is at 228 kDa.
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