IHC-plus(tm) NES / Nestin Antibody

LifeSpan BioSciences

IHC-plus(tm) NES / Nestin Antibody

23

Immunohistochemistry: LS-B656 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues and a panel of mouse tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B656 was determined to be 5 ug/ml.. ELISA (1:20000 - 1:80000), IHC (5 µg/ml), IHC-P (5 µg/ml), WB (1:500 - 1:3000) Immunohistochemistry: LS-B656 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues and a panel of mouse tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B656 was determined to be 5 ug/ml.

Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

1.23 mg/ml

Unconjugated

NES

nestin

Nbla00170, nestin

Rabbit

IgG

Human, Mouse

-20°C,2°C to 8°C

41116161