IHC-plus(tm) NR4A1 / NUR77 Antibody (aa251-266)

LifeSpan BioSciences

IHC-plus(tm) NR4A1 / NUR77 Antibody (aa251-266)

23

Immunohistochemistry: LS-B114 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B114 was determined to be 20-40 ug/ml.. ChrIP (1:10 - 1:500), ICC, IF, IHC, IHC-P (20 - 40 µg/ml), IP (1:10 - 1:500), WB (1:500 - 1:1000) Immunohistochemistry: LS-B114 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B114 was determined to be 20-40 ug/ml.

ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

1 mg/ml

Unconjugated

NR4A1

nuclear receptor subfamily 4 group A member 1

GFRP1, HMR, N10, NAK-1, NGFIB, NP10, NUR77, TR3, nuclear receptor subfamily 4immunitygroup A member 1, ST-59, TR3 orphan receptor, early response protein NAK1, growth factor-inducible nuclear protein N10, hormone receptor, nuclear hormone receptor NUR/77, orphan nuclear receptor HMR, orphan nuclear receptor TR3, steroid receptor TR3, testicular receptor 3

Rabbit

IgG

Human, Mouse, Rat

-20°C,2°C to 8°C

41116161