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ProductsBack/IHC-plus(tm) PLAT / TPA Antibody

IHC-plus(tm) PLAT / TPA Antibody

LS-B12329
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetPLAT
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) PLAT / TPA Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 32kDa/44kDa/57kDa/62kDa, while the observed MW by Western blot was 63kDa.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC (1:50 - 1:200), IHC-P (1:200), WB (1:500 - 1:2000) The predicted MW is 32kDa/44kDa/57kDa/62kDa, while the observed MW by Western blot was 63kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5327
  • Target name
    PLAT
  • Target description
    plasminogen activator, tissue type
  • Target synonyms
    T-PA, TPA, tissue-type plasminogen activator, alteplase, plasminogen/activator kringle, reteplase, t-plasminogen activator
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PLAT using anti-PLAT antibody (A02965-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U2OS whole cell lysates, Lane 3: human U-87MG whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: rat kidney tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLAT antigen affinity purified polyclonal antibody (Catalog # A02965-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLAT at approximately 63-69KD. The expected band size for PLAT is at 63KD.
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