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IHC-plus(tm) PTGER1 / EP1 Antibody

LS-B6942
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetPTGER1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) PTGER1 / EP1 Antibody
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ICC, IHC, IHC-P (1:100), WB (1:200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5731
  • Target name
    PTGER1
  • Target description
    prostaglandin E receptor 1
  • Target synonyms
    EP1, prostaglandin E2 receptor EP1 subtype, PGE receptor, EP1 subtype, PGE2 receptor EP1 subtype, prostaglandin E receptor 1 (subtype EP1), 42kD, prostaglandin E receptor 1 (subtype EP1), 42kDa, prostaglandin E receptor 1, subtype EP1, prostanoid EP1 receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Blank control (Black line): Mouse blood (Black)._x000D_ Primary Antibody (green line): PTGER1 Polyclonal Antibody (bs-6316R)_x000D_ Dilution: 3μg /10^6 cells;_x000D_ Isotype Control Antibody (orange line): Rabbit IgG ._x000D_ Secondary Antibody (white blue line): Goat anti-rabbit IgG_x000D_ Dilution: 1μg /test._x000D_ Protocol_x000D_ The cells were fixed with 4% PFA (10min at room temperature)and then were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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Figure 1. Western blot analysis of PTGER1 using anti-PTGER1 antibody (PA1583). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTGER1 antigen affinity purified polyclonal antibody (Catalog # PA1583) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTGER1 at approximately 42 kDa. The expected band size for PTGER1 is at 42 kDa.
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