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ProductsBack/IHC-plus(tm) RNF8 Antibody (aa1-260)

IHC-plus(tm) RNF8 Antibody (aa1-260)

LS-B15238
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetRNF8
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) RNF8 Antibody (aa1-260)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA, IHC, IHC-P (10 µg/ml), WB (1:500 - 1:5000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9025
  • Target name
    RNF8
  • Target description
    ring finger protein 8
  • Target synonyms
    hRNF8, E3 ubiquitin-protein ligase RNF8, C3HC4-type zinc finger protein, RING-type E3 ubiquitin transferase RNF8, UBC13/UEV-interacting ring finger protein, ring finger protein (C3HC4 type) 8, ring finger protein 8, E3 ubiquitin protein ligase
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C,-80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RNF8 using anti-RNF8 antibody (A00707-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RNF8 antigen affinity purified polyclonal antibody (Catalog # A00707-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RNF8 at approximately 56 kDa. The expected band size for RNF8 is at 56 kDa.
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Sample (30 ug of whole cell lysate) A: HepG2 7.5% SDS PAGE GTX115176 diluted at 1:5000
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