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ProductsBack/IHC-plus(tm) SAE2 / UBA2 Antibody

IHC-plus(tm) SAE2 / UBA2 Antibody

LS-B5092
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetUBA2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) SAE2 / UBA2 Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    SAE2 antibody can be used for detection of SAE2 by Western blot at 0.25 ug/mL. Antibody can also be used for immunocytochemistry starting at 4 ug/mL. For immunofluorescence start at 5 ug/mL. Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot:. ELISA, ICC, IF, IHC, IHC-P (5 µg/ml), WB (0.25 - 0.5 µg/ml) SAE2 antibody can be used for detection of SAE2 by Western blot at 0.25 ug/mL. Antibody can also be used for immunocytochemistry starting at 4 ug/mL. For immunofluorescence start at 5 ug/mL. Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot:
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID10054
  • Target name
    UBA2
  • Target description
    ubiquitin like modifier activating enzyme 2
  • Target synonyms
    ACCES, ARX, HRIHFB2115, SAE2, SUMO-activating enzyme subunit 2, SUMO-1 activating enzyme subunit 2, SUMO1 activating enzyme subunit 2, UBA2, ubiquitin-activating enzyme E1 homolog, anthracycline-associated resistance ARX, ubiquitin-like 1-activating enzyme E1B
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SAE2/UBA2 using anti-SAE2/UBA2 antibody (A03816-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: human SK-OV-3 whole cell lysates, Lane 6: human 22RV1 whole cell lysates, Lane 7: human A431 whole cell lysates, Lane 8: human COLO-320 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SAE2/UBA2 antigen affinity purified polyclonal antibody (Catalog # A03816-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SAE2/UBA2 at approximately 90KD. The expected band size for SAE2/UBA2 is at 71KD.
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