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ProductsBack/IHC-plus(tm) TOLLIP Antibody

IHC-plus(tm) TOLLIP Antibody

LS-B608
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetTOLLIP
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) TOLLIP Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    TOLLIP antibody can be used for the detection of TOLLIP by Western blot at 1 - 2 ug/mL. Antibody can also be used for immunohistochemistry starting at 5 ug/mL and immunocytochemistry starting at 2 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot:. ELISA, ICC, IF, IHC, IHC-P (3 µg/ml), WB (1 µg/ml) TOLLIP antibody can be used for the detection of TOLLIP by Western blot at 1 - 2 ug/mL. Antibody can also be used for immunohistochemistry starting at 5 ug/mL and immunocytochemistry starting at 2 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot:
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID54472
  • Target name
    TOLLIP
  • Target description
    toll interacting protein
  • Target synonyms
    IL-1RAcPIP, toll-interacting protein, adapter protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TOLLIP using anti-TOLLIP antibody (A02039-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TOLLIP antigen affinity purified polyclonal antibody (Catalog # A02039-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TOLLIP at approximately 30 kDa. The expected band size for TOLLIP is at 30 kDa.
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