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ProductsBack/IHC-plus(tm) TRIM28 / KAP1 Antibody

IHC-plus(tm) TRIM28 / KAP1 Antibody

LS-B10061
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetTRIM28
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) TRIM28 / KAP1 Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    TRIM28 antibody can be used for detection of TRIM28 by Western blot at 1 - 2 ug/mL. Antibody validated: Western Blot in human samples. All other applications and species not yet tested. Western Blot: Predicted: 92 kDa. ELISA, IHC, IHC-P (10 µg/ml), WB (1 - 2 µg/ml) TRIM28 antibody can be used for detection of TRIM28 by Western blot at 1 - 2 ug/mL. Antibody validated: Western Blot in human samples. All other applications and species not yet tested. Western Blot: Predicted: 92 kDa
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID10155
  • Target name
    TRIM28
  • Target description
    tripartite motif containing 28
  • Target synonyms
    KAP1, PPP1R157, RNF96, TF1B, TIF1B, TIF1beta, WT7, transcription intermediary factor 1-beta, E3 SUMO-protein ligase TRIM28, KAP-1, KRAB [Kruppel-associated box domain]-associated protein 1, KRAB-interacting protein 1, KRIP-1, RING finger protein 96, RING-type E3 ubiquitin transferase TIF1-beta, TIF1-beta, nuclear corepressor KAP-1, protein phosphatase 1, regulatory subunit 157, transcriptional intermediary factor 1-beta
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of KAP1 using anti-KAP1 antibody (PB9835). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse spleen tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KAP1 antigen affinity purified polyclonal antibody (Catalog # PB9835) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KAP1 at approximately 100 kDa. The expected band size for KAP1 is at 89 kDa.
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